香蕉束顶病毒NS株DNA组分5的克隆和序列分析  

Cloning and Sequence Analysis of Component 5 of Banana Bunchy Top Virus NS Strain

作　　者：郑耘[1] 阮小蕾[1] 李华平[1] 肖火根[1] 范怀忠[1] 

机构地区：[1]华南农业大学植物病毒研究室,广州510642

出　　处：《中国病毒学》2005年第3期311-314,共4页Virologica Sinica

基　　金：广东省科技攻关项目(C20209);国家自然科学基金(39870434)

摘　　要：本文利用PCR技术扩增得到香蕉束顶病毒(BBTV)NS株DNA组分5的全基因,该基因全长为1014nt,具有一个开放阅读框,编码146个氨基酸,蛋白质二级结构包括6个α螺旋,7个β折叠。NS株系与南太平洋组澳大利亚、夏威夷、埃及分离物DNA组分5核苷酸和编码的氨基酸序列相比较,核苷酸序列同源率介于88%～89%之间,氨基酸序列同源率介于80%～88%之间。NS株系与其它分离物在编码成视网膜细胞瘤蛋白(Retinoblastomaprotein,Rb)的基元序列“LXCDE”附近的二级结构上表现明显的差异,推测这种差异可能影响与植物中Rb蛋白的结合效率。The DNA component 5 (DNA-5) of Banana bunchy top virus NS strain was cloned and sequenced. It was 1014nts in full length and could code for 146 amino acids. The nucleotide and amino acid sequences of the DNA-5 were compared with those of isolates from Australia, Hawaii, Egypt, and identities of nucleotide and amino acid sequence among these isolates were between 88%~89% and 80%~88%respectively. The secondary structure near the “LXCDE” motif was much different between NS strain and others. It was deduced that efficiency of binding Rb protein would be influenced by this kind of difference.

关 键 词：香蕉束顶病毒 DNA组分5 序列分析 

分 类 号：S432.41[农业科学—植物病理学]