大野芋种子形成丛生芽的微繁殖  

Micropropagation of Colocasia gigantea by Crowded Buds from Seeds

作　　者：龙春林[1] 程治英[1] 蔡秀珍[1] 

机构地区：[1]中国科学院昆明植物研究所,云南昆明650204

出　　处：《云南植物研究》2005年第3期327-330,共4页Acta Botanica Yunnanica

基　　金：国家自然科学基金(30170102);云南省自然科学基金(2001C0058M);中国科学院(KSCX2-SW-117)

摘　　要：大野芋(Colocasiagigantea)的成熟种子(褐色)和未成熟的种子(淡黄色)在1/2MS培养基上均能萌发,种子萌发率最高达50%,种子没有休眠期。在室温下,种子在1/4MS+1%蔗糖培养基上,寿命约可达1年。种子在MS+BA2mg·L-1+IAA0.25mg·L-1的培养基上,产生丛生芽。增值率1∶4/60d。生根培养基为MS+NAA0.3mg·L-1,生根率达95%以上。通过诱导大野芋种子产生丛生芽,建成了快繁无性系,并成功地实现了种子的离体保存。本研究工作的完成,对于芋头的这一野生近缘种的保存和利用、芋头品种的改良,均具有较大意义。The mature seeds (brown) and immature seeds (light yellow) of Colocasia gigantea started to germinate after they were grown on 1/2MS media for 30 days.The germination rate was up to 50%.There is not dormancy behavior in gigantean seeds.The longevity of seeds could be almost one year if they were stored on 1/4MS+1% sugar media.The seed and seedlings with height of 1?cm could produce bud cluster on the media of MS+BA2?mg·L -1 +IAA0.25?mg·L -1 ,and the proliferation rate could be 1∶4/60?d.The media for rooting was MS+NAA0.3?mg·L -1 ,and the rooting rate could be over 95%.The successful induction of bud clusters in vitro from seeds would be valuable in Colocasia breeding and cultivation,and selection of fine clone of C.gigantea ,and exploration,utilization and protection of C.gigantea resources.

关 键 词：大野芋 丛生芽 微繁殖 种子 

分 类 号：S682.36[农业科学—观赏园艺]