病毒性脑炎患者Borna病病毒P24基因片段的检测  

Detection of Borna disease virus P24 fragment from peripheral blood cells in patients with viral encephalitis

作　　者：徐平[1] 谢鹏[1] 邹德智[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科

出　　处：《中华神经科杂志》2005年第5期309-312,共4页Chinese Journal of Neurology

基　　金：国家自然科学基金资助项目(39870252)

摘　　要：目的探讨Borna病病毒(BDV)感染与人类病毒性脑炎的关系。方法采用荧光定量套式逆转录酶聚合酶链反应(FQ nRT PCR)检测了59例临床诊断的原因未明的病毒性脑炎及112名健康人外周血单个核细胞(PBMC)中BDVP24基因片段。结果59例原因未明的病毒性脑炎患者中有3例PBMC中检出BDVP24基因片段,而112名健康对照均未检出。病毒性脑炎患者BDV阳性率(5.08%)高于健康对照,差异有统计学意义(P<0.05),且BDVP24基因片段检测阳性病例的脑脊液中其他常见致脑炎病毒(单纯疱疹病毒、带状疱疹病毒、腮腺炎病毒、柯萨奇病毒和巨细胞病毒)检查均为阴性。结论中国的部分病毒性脑炎患者中存在BDV感染,BDV感染与病毒性脑炎的发病可能有关。ObjectiveTo study the relationship between Borna disease virus (BDV) and the human viral encephalitis.MethodsThe P24 fragment of BDV RNA in peripheral blood mononuclear cells (PBMC) from 59 patients with viral encephalitis diagnosed clinically, and 112 healthy donors were examined by fluorescence quantitative nested reverse transcriptase polymerase chain reaction (FQ-nRT-PCR).ResultsThere were 3 positive of BDV P24 fragment in 59 patients with viral encephalitis, and no positive in blood donors. The positive rate of BDV p24 in PBMC in viral encephalitis (5.08%) was higher significantly than that in blood donors (P<0.05). Furthermore, other common viruses caused viral encephalitis (such as herpes simplex virus, herpes zoster virus, mumps virus, Coxsackie virus, and cytomegalovirus, etc.) could not be detected positively by PCR in cerebrospinal fluid of these 3 cases presented BDV P24 fragment positive.ConclusionThe infection of BDV should present in patients with viral encephalitis in China, and might be the cause of viral encephalitis.

关 键 词：病毒性脑炎 基因片段 BORNA病病毒 患者 检测 套式逆转录酶聚合酶链反应 P24 人外周血单个核细胞 Boma病病毒 单纯疱疹病毒 带状疱疹病毒 原因未明 腮腺炎病毒 巨细胞病毒 柯萨奇病毒 BDV 临床诊断 荧光定量 PBMC 脑炎病毒 

分 类 号：R512.3[医药卫生—内科学]