CD2相关蛋白mRNA在大鼠阿霉素肾病模型中的表达  

Expression of CD2AP mRNA in rats with adriamycin nephropathy

作　　者：何伟春[1] 邢昌赢[1] 吴晓蓉[1] 钱军[1] 赵秀芬[1] 

机构地区：[1]南京医科大学第一附属医院肾内科,210029

出　　处：《江苏医药》2005年第6期454-456,i003,共4页Jiangsu Medical Journal

基　　金：江苏省135工程重点医学人才基金资助(RC2002045)

摘　　要：目的观察CD2相关蛋白(CD2AP)mRNA在大鼠阿霉素肾病模型中的表达,探讨其在蛋白尿发生发展过程中的作用。方法建立大鼠阿霉素肾病模型,应用光镜、电镜观察不同时期(第2、4、6、8周末)肾组织的病理改变,并采用半定量RTPCR的方法,检测大鼠肾皮质中CD2APmRNA的表达。结果阿霉素注射后:(1)大鼠24h尿蛋白量逐渐增加,第2、4、6、8周末分别为(144.5±16.4)、(250.0±45.5)、(440.1±62.1)和(626.5±94.2)mg/24h,均显著高于正常对照组(24.3±6.5)mg/24h(P<0.001);(2)大鼠肾皮质CD2APmRNA的表达在第4、6、8周末分别达到正常对照组的1.7倍、2.5倍和1.9倍。CD2APmRNA的表达与尿蛋白量呈正相关(r=0.58,P<0.01)。结论阿霉素肾病模型大鼠肾皮质CD2APmRNA的表达与大鼠蛋白尿的发生发展具有一定的相关性。Objective To investigate the expression of CD2-associated protein (CD2AP) mRNA in rats with adriamycin nephropathy, and to explore the possible effect on the development of proteinuria.Methods Thirty SD rats, weighted from 140 to 160g,were randomly divided into the nephropathy model groups and normal control group. The nephropathy model was established by a single intravenous injection of adriamycin 6mg/kg.While the normal control group received the same volume of 0.9% sodium chloride. At the end of the 2 nd, 4 th, 6 th or 8 th week after injection, the rats were sacrificed and the kidneys were acquired. The renal histology was observed under light microscope and electronic microscope. The semiquantitative RT-PCR was used for detecting the levels of CD2AP mRNA.Results (1)CD2AP mRNA levels in the 4th,6th and 8th week groups were 1.7, 2.5 and 1.9 times those in normal control group respectively.(2)The expression of CD2AP mRNA was positively correlated with the amount of proteinuria (r=0.56,P<0.01).Conclusion The increased expression of CD2AP mRNA is to some extent related to the development of proteinuria in adriamycin nephropathy rats.

关 键 词：肾病模型 蛋白MRNA 半定量RT-PCR CD2AP 正常对照组 发生发展 尿蛋白量 阿霉素肾病 肾皮质 相关蛋白 方法建立 不同时期 电镜观察 病理改变 模型大鼠 蛋白尿 24h 肾组织 周末 注射后 正相关 相关性 

分 类 号：R692.6[医药卫生—泌尿科学] R735.7[医药卫生—外科学]