枳椇子提取物对肝纤维化保护作用的形态学研究  

A Morphological Study of Protective Effect of Hovenia Dulcis Thunb Extract on Hepatic Fibrosis

作　　者：叶丽萍[1] 张洪[1] 刘秀琳[1] 

机构地区：[1]武汉大学人民医院药学部,武汉430060

出　　处：《武汉大学学报（医学版）》2005年第3期293-296,i005,共5页Medical Journal of Wuhan University

基　　金：湖北省科技攻关项目(编号:2002AA301C61)

摘　　要：目的:研究枳椇子提取物对四氯化碳(CCl4)诱导的实验性肝纤维化的保护作用。方法:用CCl4制备大鼠肝纤维化模型,分别取组织于光镜电镜下观察。结果:模型组肝细胞大量坏死,汇管区炎性细胞浸润,假小叶形成,线粒体肿胀,膜不清晰,嵴消失,内质网扩张明显,溶酶体减少。预防组能减轻纤维化、肝细胞的变性坏死及炎性细胞浸润,使线粒体膜及嵴恢复,减少内质网扩张,促进细胞再生,阻滞纤维化的发生发展。结论:枳椇子具有明确抗纤维化作用,其机理可能与减轻有毒物质对肝细胞的损伤,拮抗细胞脂质过氧化,稳定细胞膜有关。Objective: To investigate the protective effect of the Hovenia dulcis Thunb extract on the carbon tetrachloride (CCl 4) induced experimental hepatic fibrosis in rats. Methods: CCl 4 was used to induce liver fibrosis in Wistar rats. 8 weeks later, tissues were taken for the detection under microscope and electron microscope. Results: In the model group, hepatic cells degenerated and became necrotic, inflammatory cell infiltrated,and pseudolobular came into being. The mitochondria swelled and its cristedis disappeared, and the endoplasmic reticulum dilated. In the prevented group, hepatic cells degeneration and necrosis was in slight extent, mitochondria membrane and cristedis resumed, dilatation of the endoplasmic reticulum decreased, and the extract facilitated the regeneration of the hepatocyte, and retarded the forming of liver fibrosis. Conclusion: Hovenia dulcis Thunb can definitely prevent liver fibrosis. The possible mechanism is to reduce hepatotoxic respones, anti-peroxidation of lipid, and stabilization of cell membrane.

关 键 词：肝纤维化 枳棋子 形态学 

分 类 号：R285.5[医药卫生—中药学]