RGD改性聚醚氨酯及其内皮细胞相容性的研究  

Surface Modification of Poly(ether urethane) Membrane with RGD and Its Cytocompatibility of Human Endothelial Cell

作　　者：余贯华[1] 计剑[1] 王东安[1] 沈家骢[1] 

机构地区：[1]浙江大学高分子科学与工程学系,杭州310027

出　　处：《高等学校化学学报》2005年第6期1156-1161,共6页Chemical Journal of Chinese Universities

基　　金：国家重点基础研究发展规划项目(批准号:G1999054305)资助.

摘　　要：利用氢键稳定的溶液互穿技术对聚醚氨酯(PEU)进行改性.用ATR-FTIR对十八烷基-聚氧乙烯-4,4′-二苯甲烷二异氰酸酯-聚氧乙烯-十八烷基(MSPEO)与PEU共混膜表面进行研究,结果表明,MS-PEO中的氨基甲酸酯链段与PEU基材之间发生了氢键缔合的作用.通过水化处理PEO及十八烷基自发地富集在基材表面.根据氢键缔合和表面自迁移原理,设计了两种RGD改性聚醚氨酯的方法:(1)将含RGD端基的聚氧乙烯-4,4′-二苯甲烷二异氰酸酯-聚氧乙烯偶联物(MPEO-RGD)与PEU进行共混改性,利用RGD端基及PEO的自迁移特性获得RGD富集的表面;(2)将含甲磺酸酯端基的聚氧乙烯-4,4′-二苯甲烷二异氰酸酯-聚氧乙烯偶联物(MPEO-mesyl)与PEU共混成膜,并对膜片进行水化处理,使甲磺酸酯端基富集在PEU表面,浸泡于RGD的PBS溶液中,在膜片表面成功地原位接枝了RGD.对两种RGD改性方法获得的表面进行了内皮细胞的培养,结果表明,两种改性方法均大大提高了PEU的细胞相容性,其中方法(1)共混改性的表面细胞相容性略优于方法(2)的接枝改性表面.X\_PEO-MDI-PEO\_X'-type pentablock coupling copolymers were specially designed as the surface additives for poly(ether urethane)(PEU). When the pentablock coupling polymers were blended with PEU, ATR-FTIR indicates that the middle blocks(diphenylmethane diisocyanate(MDI) of the pentablock copolymers could incorporate with the hard blocks of PEU chains through hydrogen binding, which improved the stability of the 'X\_PEO-MDI-PEO\_X'. Based on the special hydrogen binding stabilization and self-migration of 'X\_PEO-MDI-PEO\_X', RGD presenting surfaces were developed via two different methods. The first approach involved directly blending of RGD-tethered poly(ethylene oxide)-MDI-poly(ethylene oxide)-RGD in PEU. In the second approach, the mesyl-PEO-MDI-PEO\_ mesyl were non-covalently introduced onto the PEU surfaces by dip coating, upon which the RGD was covalently immobilized in situ by cleavage of the original mesyl end groups. The human endothelial cell tests indicate that the RGD presenting films modified by both method could promote endothelial cells attachment and growth significantly. And the cytocompatibility of PEU modified by the first method was better than that by the second method. The two simple methods of surface treatment may have a potential for tissue engineering and other biomedical applications.

关 键 词：氢键 RGD 聚醚氨酯 表面改性 细胞相容性 

分 类 号：O631[理学—高分子化学]