石英晶体微天平实时监测低密度脂蛋白在胆固醇修饰葡聚糖上的吸附及其动力学研究  

Monitoring Adsorption in situ and Kinetic Studies of Low-density Lipoprotein onto Cholesterol Modified Dextran Using Quartz Crystal Microbalance

作　　者：韩松岩[1] 刘东波[1] 黄亮[1] 王深琪[2] 刘清平[2] 安齐顺一[3] 长哲郎[3] 陈强[1] 

机构地区：[1]南开大学生命科学学院,天津300071 [2]南开大学生物活性材料教育部重点实验室,天津300071 [3]日本东北大学大学院药学研究科,仙台9808578

出　　处：《高等学校化学学报》2005年第6期1166-1169,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:90209054);国家"八六三"项目(批准号:2002AA326060);南开大学;天津大学科技合作基金项目资助.

摘　　要：利用石英晶体微天平实时监测低密度脂蛋白(LDL)在胆固醇修饰葡聚糖(CMD)上的吸附,并对其吸附动力学进行研究.结果表明,CMD与LDL之间的相互作用符合Langmuir吸附方程,当LDL浓度(ng/μL)为9.9,12.38和14.14时,其吸附平衡常数[K/(mol·L-1·s-1)]分别为0.0477,0.0536和0.0628,表面吸附量(ng/cm2)分别为107.6,139.6和167.9.最大吸附量达到284.8ng/cm2,吸附率为72.91%.The use of quartz crystal microbalance(QCM) for monitoring in situ the adsorption of low-density lipoprotein(LDL) onto cholesterol modified dextran(CMD) was described. The kinetic studies were made. The experimental results show that kinetic curves are fitted for that of Langmuir adsorption equation quite well, when LDL mass concentration(ng/μL) are 9.9, 12.38, 14.14, respectively, and the rate constants for adsorption(mol·L^(-1)·s^(-1)) and adsorption capacity(ng/cm^2) are estimated to be (0.047 7), 0.053 6, 0.062 8 and 107.6, 139.6, 167.9, respectively. The maximal LDL adsorption capacity attains 284.8 ng/cm^2 and the adsorptive efficiency was calculated as 72.91%.

关 键 词：石英晶体微天平 胆固醇修饰葡聚糖 低密度脂蛋白 吸附 

分 类 号：O636[理学—高分子化学]