大鼠视网膜变性中药保护作用的实验研究  

Study in the protective effect of traditional Chinese medicines on induced retinal degeneration in rats

作　　者：任京力[1] 杨锦南[1] 付佶迒[2] 

机构地区：[1]新乡医学院药学院,河南省新乡市453003 [2]新乡医学院第一临床医院病理科,河南省卫辉市453100

出　　处：《眼科新进展》2005年第3期209-211,共3页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30300467)河南省科学技术厅重点攻关项目(No:0523030700)~~

摘　　要：目的观察金钠多(Ginaton,Gin)和葛根素(Puerarin,Pue)对N-甲基-N-亚硝脲(N-methyl-N-nitrosorea,MNU)诱导SD大鼠视网膜变性的保护作用及机制。方法雌性SD大鼠随机分为正常对照组、模型组、Gin大剂量和中剂量组、Pue大剂量和中剂量组。于生后47d开始腹腔注射给药,每日1次。并于生后50d,模型组和各药物处理组的大鼠腹腔注射MNU60mg·kg-1,正常对照组腹腔注射生理盐水。在MNU或生理盐水处理后不同时间处死动物,取眼球。视网膜形态学分析测量周边视网膜总厚度,TUNEL试剂盒检测感受器细胞凋亡。结果MNU处理7d后,模型纽周边视网膜总厚度为36μm,Gin组(大剂量和中剂量)和Pue组(大剂量和中剂量)分别为(44±2)μm,(37±2)μm,(46±2)μm,(35±2)μm。MNU处理24h后,模型组周边视网膜光感受细胞凋亡指数为(38.0±3.6)%,Gin大剂量组、中剂量组、Pue大剂量组和中剂量组分别为(26.3±2.7)%,(37.4±2.9)%,(25.4±3.0)%,(39.0±2.5)%.结论Gin和Pue对MNU引起周边视网膜损伤有一定的保护作用,呈剂量依赖性,其作用机制是通过抑制光感受器细胞发生凋亡。但二者对中心视网膜均无保护作用。Objective To observe the protective effects of Ginaton (Gin) and Puerarin (Pue) on N-methyl-N-nitrosourea (MNU)-induced retinal degeneration in SD rats and to explore its mechanisms. Methods Female SD rats were divided into 6 groups randomly, including control group, model group, puerarin groups (high dose and medium dose)and ginaton groups (high dose and medium dose). Drugs were injected intraperitoneally into 47-day SD rats once a day. At 50 days of age,all rats in model group and drug groups also received a single intraperitoneal injection of 60 mg±kg-1 MNU. Rats in control group received i. p. injection of physiological saline. All rats were sacrificed at different times after MNU or physiological saline treatment. The apoptotic index of photoreceptor cells were calculated by TUNEL labeling and retinal damage was evaluated based on retinal thickness. Results At the 7th day after MNU treatment, the peripherial retinal thickness was 36 μm in model group, (44 ± 2) fan and (37± 2) fan in high and medium doses of Gin groups, (46 ± 2) fun and (35±2)μm in high and medium doses of Pue groups, respectively. In the peripheral retina, the apoptotic index 24 hours after MNU treatment was(38.0±3.6)% in model group, (26.3±2.7)% and (37.4±2.9)% in high and medium doses of Gin groups, (25.4±3.0) % and (39.0± 2.5) % in high and medium doses of Pue groups. Conclusion Gin or Pue could effectively suppress MNU-induced peripheal retinal damage by anti-apoptosis in a dose-dependent manner. However,no protective effect on MNU-induced central retinal damage was found.

关 键 词：N-甲基-N-亚硝脲 金钠多 葛根素 视网膜 大鼠 

分 类 号：R965.1[医药卫生—药理学]