吡那地尔对烫伤大鼠心肌线粒体结构及呼吸功能的影响  

Influence of pinacidil on the myocardial mitochondrial structure and the respiratory function in scalded rats

作　　者：彭念寅[1] 周红[2] 司良毅[1] 

机构地区：[1]第三军医大学西南医院老年病科,重庆400038 [2]第三军医大学药理学教研室,重庆400038

出　　处：《中华烧伤杂志》2005年第3期170-172,共3页Chinese Journal of Burns

基　　金：国家重点基础研究发展规划资助项目(G1999054502)

摘　　要：目的　观察吡那地尔预处理后对烫伤大鼠心肌线粒体结构及呼吸功能的影响。方法将75只健康Wistar大鼠随机分为对照组(9只,腹腔注射50μg/kg等渗盐水)、单烫组(33只,仅作烫伤)及预处理组(33只,腹腔注射吡那地尔50μg/kg后再作烫伤)。透射电镜观察3组大鼠心肌线粒体结构的变化,用生物氧特性微机测量分析系统检测大鼠线粒体的呼吸控制率,并检测大鼠心肌线粒体中丙二醛(MDA)和超氧阴离子的含量。　结果　伤后3h预处理组大鼠心肌线粒体病理损害程度较单烫组轻;伤后1—6h其呼吸控制率显著高于单烫组(P<0. 05或0. 01);MDA和超氧阴离子含量分别于伤后3、6h和伤后1、3、6h低于单烫组(P<0. 05或0. 01)。伤后3h预处理组MDA、超氧阴离子含量各为(0. 60±0. 09 )μmol/g、0. 127±0. 020, 明显低于单烫组( 0. 83±0. 07 )μmol/g、0 169±0. 015(P<0. 01).　结论　吡那地尔预处理对烫伤大鼠心肌产生了明显的保护作用,其机制可能与线粒体中对腺苷三磷酸敏感的钾离子通道提前开放有关。Objective To investigate the influence of pinacidil preconditioning on the protection of the structure and respiratory function of injured myocardial mitochondria in scalded rats. Methods Seventy-five healthy Wistar rats, weighed 250～300 g, were randomly divided into three groups: i.e. control(n=9,with intraperitoneal injection of 50 μg/kg isotonic saline), scald (n=33, with 30% TBSA full thickness scald) and pre-conditioning (n=33, with same extent of scald injury after intraperitoneal injection of 50 μg/kg pinacidil) groups. Mitochondrial ultrastructure was observed by transmission electron microscope. The mitochondrial respiratory function, the MDA content and the superoxide anion level were determined with corresponding methods. Results The degree of injury to rat myocardial mitochondria in pre-conditioning group was less intensive than that in scald group( P <0.05 or 0.01). The respiratory control rate in pre-conditioning group was obviously higher than that in scald group ( P <0.05),and the contents of MDA and superoxide anion in pre-conditioning group were markedly lower than those in scald group( P <0.05 or 0.01), as evidenced by their contents at 3 post scalding hours (0.60±0.09 μmol/g and 0.127±0.020) were obviously lower than those in scald group(0.83±0.07 μmol/g and 0.169±0.015)( P <0.01). Conclusion Pinacidil preconditioning was beneficial in the protection of myocardial mitochondria in scalded rats, and it might be related to the pre-opening of potassium channel which was sensitive to mitochondrial ATP.

关 键 词：线粒体结构 大鼠心肌 吡那地尔 呼吸功能 烫伤 Wistar大鼠 丙二醛(MDA) 呼吸控制率 心肌线粒体 透射电镜观察 病理损害程度 腹腔注射 0.05 离子含量 预处理 超氧阴离子 钾离子通道 腺苷三磷酸 系统检测 测量分析 保护作用 

分 类 号：R96[医药卫生—药理学]