烧伤血清对骨髓红系及粒系造血功能影响的实验研究  

The effects of burn serum on the erythropoiesis and granulopoiesis in bone marrow in mice

作　　者：周燕虹[1,2] 罗成基[1] 郭朝华[1] 孔佩艳[1] 邹仲敏[1] 

机构地区：[1]第三军医大学全军复合伤研究所,创伤,烧伤与复合伤国家重点实验室,重庆400038 [2]第三军医大学卫生毒理学教研室

出　　处：《中华烧伤杂志》2005年第3期177-180,共4页Chinese Journal of Burns

基　　金：全军"九五"医药卫生攻关课题资助项目(96L045)

摘　　要：目的　观察烧伤血清对正常小鼠骨髓红系、粒系造血功能的影响,初步探讨其可能的机制。　方法　常规制备小鼠骨髓细胞(BMC),用其分别建立红系集落形成单位(CFU -E)培养体系和粒单核细胞集落形成单位(CFU -GM)培养体系。在两体系中均加入15%TBSAⅢ度烧伤小鼠伤后12h及1、3、5、7、10d的血清(烧伤血清组)和小鼠正常血清(正常血清组),另设阳性对照组和空白对照组,检测各血清对两培养体系的刺激活性。用放射免疫法检测烧伤血清中红细胞生成素(EPO)和粒巨噬细胞集落刺激因子(GM -CSF)浓度的变化,并将此二指标分别与烧伤血清对CFU- E、CFU -GM培养体系的刺激活性作对数线性拟合相关分析。　结果　(1)烧伤血清对CFU- E、CFU -GM培养体系的刺激活性明显升高,均在伤后1d达峰值[ (384±60)、(127±16)CFU](P<0. 01),此后逐渐下降,到伤后7d[(125±14)、(34±20)CFU]接近正常血清组水平(P>0. 05). (2)烧伤血清中EPO浓度在伤后12h—7d较正常值显著升高(P<0. 01);GM -CSF浓度在伤后12h、1d显著高于正常值(P<0. 05). (3)烧伤血清EPO浓度与烧伤血清对CFU- E培养体系的刺激活性呈显著对数正相关(r=0.857 0,P=0. 013 7);GM- CSF浓度与烧伤血清对CFU GM培养体系的刺激活性无显著相关性(r=0.704 9,P>0. 05).　结论　小鼠烧伤?Objective To observe the effects of burn serum on the erythropoiesis and granulopoiesis in bone marrow in mice,and to explore the possible underlying mechanism. Methods Murine bone marrow cell (BMC) strain was prepared routinely and was employed in the establishment of the culture system of colony forming units of erythrocytes, or granulocytes and monocytes. To both sets of culture system normal murine serum (N group) and burn serum ,which was collected from the mice with 15% full thickness burn at 12 postburn hours (PBH) and 1,3,5,7 and 10 postburn days (PBD),(burn serum group) was added . In addition, positive control and blank control groups were set accordingly. The stimulating activity of all kinds of sera on the BMCs in the two sets of culture system was determined. The changes in the burn serum concentrations of EPO and GM-CSF were detected by radioimmunoassay, and the data were analyzed by logarithmic linear fitting correlation with the former influence of burn sera on the erythrocytes and granulocytes.Results (1) Burn sera exhibited obvious stimulation promoting activity on the erythropoiesis and granulopoiesis in BMC, and the activity peaked (384±60 and 127±16 CFU) on 1 PBD and decreased thereafter to approach the values found in normal sera group (125±14 and 34±20 CFU) on 7 PBD. (2) The EPO content in burn serum was evidently higher than the normal value ( P <0.01) during 12 PBH to 7PBD period. The GM-CSF concentration was obviously higher than the normal value ( P <0.05) at 12 PBH and on 1 PBD.(3) The EPO concentration in burn serum was significantly and logarithmically correlated with the stimulation promoting activity of burn serum on erythropoiesis ( r =0.8570, P =0.0137). But the GM-CSF concentration in culture with burn serum was not correlated with the stimulation promoting activity of burn serum on granulopoiesis ( r =0.7049, P >0.05). Conclusion The sera harvested from burned mice during early postburn stage exhibited strong stimulation promoting activity on the erythropoiesis and

关 键 词：烧伤血清 骨髓红系 实验研究 功能影响 粒-巨噬细胞集落刺激因子 CFU-GM GM-CSF 红系集落形成单位 粒系刺激活性 CFU-E 培养体系 粒系造血功能 小鼠骨髓细胞 粒-单核细胞 正常血清 红细胞生成素 阳性对照组 免疫法检测 

分 类 号：R644[医药卫生—外科学] R692.502[医药卫生—临床医学]