胶原凝胶复合CPPf/PLLA支架与软骨细胞的相容性研究  

Cellular compatibility study of collagen gel compounded CPPf/PLLA scaffold and chondrocytes.

作　　者：李忠[1] 杨柳[1] 陈光兴[1] 林炎水[1] 段小军[1] 

机构地区：[1]第三军医大学西南医院关节外科中心,重庆400038

出　　处：《四川医学》2005年第6期591-592,共2页Sichuan Medical Journal

基　　金：国家自然科学基金资助项目(30270375);重庆市重点科技攻关基金资助项目(2003136)

摘　　要：目的研究软骨细胞与胶原凝胶复合CPPf/PLLA支架的细胞相容性,为软骨组织工程提供适宜的种子细胞载体。方法将胶原凝胶包埋的软骨细胞整合入CPPf/PLLA三维支架并进行体外培养,采用倒置相差显微镜和扫描电镜观察细胞在支架上粘附、生长和增殖等情况,从组织学和Ⅱ型胶原免疫组化对复合组织进行评价。结果软骨细胞能良好地在支架上粘附、生长和增殖,新形成的组织为透明软骨样组织、细胞外基质有Ⅱ型胶原分布。结论胶原凝胶复合CPPf/PLLA支架具有良好的细胞相容性,可作为软骨细胞载体。Objective To study the cellular compatibility of collagen gel compounded CPPf/PLLA scaffold and Chondrocytes in order to explore for appropriate scaffold materials for cartilage tissue engineering.Methods Chondrocytes encapsulated in collagen gel were incorporated into CPPf/PLLA scaffold and cultured in vitro,then the status of adhesion,growth and proliferation of the cells were observed by inverted phase contrast microscope and scanning electron microscope;The specimens were analyzed by histology and type Ⅱ collagen immunohistochemistry.Results Chondrocytes were able to attach,grow and proliferate well on the scaffolds and chondrocytes-scaffold complexes can form cartilage-like tissue with distribution of type Ⅱ collagen in extracellular matrices.Conclusion Combination collagen gel with CPPf/PLLA scaffold exhibits excellent cellular compatibility and can be used as a vehicle for chondrocytes.

关 键 词：软骨细胞 组织工程 支架 细胞相容性 

分 类 号：R318.0[医药卫生—生物医学工程]