蝘蜓消化道5-羟色胺细胞的免疫组织化学定位  

Immunohistochemical Localization of 5-HT Cells in the Digestive Tract of Sphenomorphus iudieus

作　　者：鲍明升[1] 黄徐根[2] 吴孝兵[2] 

机构地区：[1]蚌埠医学院细胞生物学教研室,蚌埠233003 [2]安徽师范大学生命科学学院,芜湖241000

出　　处：《动物学杂志》2005年第3期20-24,共5页Chinese Journal of Zoology

基　　金：安徽省自然科学基金 (No.0 10 4 35 0 1) ;重要生物资源保护与利用安徽省重点实验室专项基金 ;安徽省学术与技术带头人专项基金 ;安徽省优秀青年基金 (No .0 4 0 4 30 4 9) ;安徽师范大学青年基金

摘　　要：应用5 羟色胺(5 Hydroxytryptamine 5 HT)特异性抗血清对蜓(Sphenomorphusiudieus)消化道5 HT细胞的分布及形态进行了免疫组织化学研究。结果表明,5 HT阳性细胞在蜓消化道各段均有分布,其中以胃幽门部位分布密度最高,食道与直肠部位其次,空肠部位分布密度最低。消化道各段5 HT细胞形态多样,有圆形、椭圆形、梭形、楔形、不规则形,其中梭形细胞多具有胞突。文中对蜓消化道5 HT细胞的分布、形态与功能相适应的特点进行了讨论。Distribution and morphology of 5-hydroxytryptamine (5-HT)cells in the digestive tract of Sphenomorphus iudieus were investigated with specific anti-5-HT antiserum by using immunohistochemical method. The results showed that 5-HT positive cells distributed throughout the digestive tract with the highest density in the pylorus, and the second highest density in the oesophagus and rectum, and the lowest density in the jejunum. The 5-HT cells were in various shapes, such as round, oval, spindle-like, wedge-like or irregular in shape.Most spindle-like cells had processes. The results suggest that the distribution and morphology of 5-HT cells in the digestive tract are likely consistent with their function in S. iudieus.

关 键 词：蝘蜓 消化道 5-羟色胺细胞 免疫组织化学 

分 类 号：Q954[生物学—动物学]