胰岛素治疗对2型糖尿病大鼠肝脂质含量及胰岛素抵抗的影响  

Effects of insulin treatment on intracellular lipid content in livers and insulin resistance in type 2 diabetic rats

作　　者：王咏波[1] 陈璐璐[1] 周慜[1] 王保平[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院内分泌科,武汉430022

出　　处：《中华肝脏病杂志》2005年第6期451-454,共4页Chinese Journal of Hepatology

基　　金：湖北省自然科学基金(2003ABA143)

摘　　要：目的探讨胰岛素治疗对2型糖尿病大鼠肝内脂质含量及胰岛素抵抗的影响。方法尾静脉注射小剂量链脲佐菌素(2 5 mg/kg)+高热量饮食建立2型糖尿病大鼠模型,根据口服葡萄糖耐量试验(OGTT) 和胰岛素分泌试验(IST)结果将其分为糖尿病非治疗(DC)组和糖尿病胰岛素治疗(DI)组,并设立正常对照(N C)组。胰岛素和等渗盐水干预4周后再行OGTT和IST,并检测各组大鼠的血脂、肝脂质及肝组织学改变。采用胰岛素敏感性指数(ISI)来评价胰岛素抵抗。结果D C组血脂、肝脂质含量明显高于NC 组(t值为2.59～1 5.77,P<0.05),而ISI则低于NC组(t=3.1 6,P<0.05),并且肝组织切片中出现明显的脂肪滴;与DC组相比,D I组血脂降低,而肝脂质显著增加(甘油三酯、胆固醇、游离脂肪酸分别增加55.70%,19.87%,22.20%),病理组织学检查显示脂肪滴增大融合,但是ISI变化不明显。结论2型糖尿病大鼠胰岛素治疗纠正血糖的同时增加了肝细胞内脂质的含量,但对胰岛素抵抗的影响不明显。Objective To study effects of insulin treatment on intracellular lipid content in livers and insulin resistance of type 2 diabetic rats. Method Type 2 diabetic rats were induced by injecting streptozotocin (25 mg/kg) and fat rich food. Then according to the results of the oral glucose tolerance test (OGTT) and glucose-induced insulin secretion test (IST), the rats were divided into two groups: control group (DC) and insulin treated group (DI). Normal rats (NC) served as controls. The treatment of each group with either NPH insulin (4~6 U · kg-1 · d-1), or saline continued for 4 weeks. Body weight, OGTT, IST, blood lipids, intracellular lipids in liver and liver histology were studied. The insulin sensitivity index (ISI) was applied to assess the status of insulin resistance. Results Blood lipid and intracellular lipids in livers in the DC were higher than those in NC (t = 2.59~15.77, P < 0.05) and the ISI was lower (t = 3.16, P < 0.05), with many fatty droplets appearing in the livers. In comparison to DC, DI showed that blood lipids were decreased, but lipids in livers were markedly increased (TG, TC, FFA increased 55.7%, 19.87%, 22.2%, respectively), and fatty droplets in hepatocytes were larger, but the ISI did not change significantly. Conclusion Insulin treatment can make blood glucose normal, increase the intracellular lipid content in the liver, and not increase the insulin resistance significantly.

关 键 词：胰岛素抵抗 胰岛素治疗 脂质含量 对2型糖尿病 口服葡萄糖耐量试验(OGTT) 胰岛素敏感性指数 病理组织学检查 糖尿病大鼠 鼠肝 高热量饮食 链脲佐菌素 尾静脉注射 组织学改变 肝组织切片 游离脂肪酸 细胞内脂质 大鼠模型 

分 类 号：R587.1[医药卫生—内分泌] R442.5[医药卫生—内科学]