肉桂油治疗小鼠CVB_(3m)病毒性心肌炎的实验研究  

Effect of Oleum Cinnamomi on mice viral myocarditis caused by CVB_(3m)

作　　者：丁媛媛[1] 谢艳华[1] 缪珊[1] 廖博[2] 王四旺[1] 

机构地区：[1]第四军医大学药学系药物研究所,陕西西安710033 [2]第四军医大学唐都医院骨科,陕西西安710038

出　　处：《第四军医大学学报》2005年第11期1037-1040,共4页Journal of the Fourth Military Medical University

摘　　要：目的:研究肉桂油(OC)对柯萨奇病毒B3m(CVB3m)诱发性小鼠病毒性心肌炎(VMC)的治疗作用.方法:0.1mLCVB3mip建立BALB/c小鼠VMC模型.正常对照组同法接种0.1mL不含病毒的Eagle液,与感染病毒小鼠隔离饲养.于接种病毒72h后给药,OC治疗组(49.1,36.7,26.5mg/kg肉桂油,ig)、阳性药物组(50mg/kg黄芪注射液,ip)、模型组与正常对照组(2500mg/kg生理盐水,ig),连续给药1wk.观察指标:累计死亡率与中位生存时间;接种病毒后10d心肌组织光、电镜组织病理学检查;心肌匀浆MDA含量、SOD活性;血清中乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酸激酶同工酶(CKMB)含量;21d心肌组织光镜病理检查.结果:OC49.1和36.7mg/kg治疗组可降低小鼠死亡率,延长中位生存时间,降低急性期血清中CK,CKMB含量以及心肌中MDA含量,提高SOD活性;减轻急性期、亚急性期小鼠心肌组织的坏死与钙化,与模型组比较差异显著(P<0.05).结论:OC具有治疗柯萨奇病毒B3m诱发性小鼠VMC的作用.AIM: To investigate the therapeutic effect of Oleum Cinnamomi(OC), volatile oil of cassia bark, on mice viral myocarditis caused by coxsackie virus B 3m(CVB 3m). METHODS: Male BALB/c mice were randomly divided into 6 groups: 1 control group (n=16)treated with normal saline i.p, 1 model group (n=30) treated with normal saline, 1 RA group (n=30) treated with 50 mg/kg Radix astragali injection i.p. as positive control and 3 treatment groups (30 in each group) respectively given 49.1, 36.7 and 26.5 mg/kg OC after 72 h viral inoculation. Except for the control group, the other 5 groups were all inoculated with CVB 3m i.p. and were bred in insulation lab. After one week treatment, the death rate, the heart histopathologic changes, the content of CK, CK-MB and LDH in mice serum, and MAD and SOD in myocardium were observed. RESULTS: The death rate, the histopathologic score, the content of CK and CK-MB in mice serum and MAD in myocardium were lower and the activities of SOD were higher in 49.1 and 36.7 mg/kg OC groups than in model group (P<0.05). The death rate was lower (P<0.05) and the histopathologic changes (necrosis, degeneration and cellular infiltration) were milder in OC groups than in model group at 21 d after viral inoculation. CONCLUSION: OC has a significant therapeutic effect on treatment of viral myocarditis caused by CVB 3m.

关 键 词：病毒性心肌炎 柯萨奇病毒B3M 肉桂油 肌酸激酶同工酶 

分 类 号：R285.5[医药卫生—中药学]