荧光检测异丙酚血药浓度  

Fluorescence detection of propofol drug concentration in plasma

作　　者：路丽华[1] 张惠 高蓉[1] 尉琳琳[1] 吴红[1] 李晓晔[1] 

机构地区：[1]第四军医大学药学系化学教研室 [2]第四军医大学口腔医院麻醉科,陕西西安710033

出　　处：《第四军医大学学报》2005年第11期1041-1042,共2页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30300456)

摘　　要：目的:探讨异丙酚血药浓度测定的荧光检测方法.方法:在含异丙酚的人体血浆样品中加入磷酸缓冲液及环己烷/正丁醇,振摇、离心,取上清液测荧光强度(激发波长λex=265nm,发射波长λem=310nm).结果:异丙酚标准液在浓度为0.1～15.0mg/L的范围内,其荧光强度与质量浓度间具有线性关系(r=0.9997).异丙酚的平均加样回收率大于95%,日内和日间RSD均小于3%.结论:该方法简便、准确、灵敏度高,可用于临床异丙酚血药浓度检测.AIM: To study the use of fluorescence detection in determining the propofol drug concentration in plasma. METHODS: Buffer solution of phosphoric acid and cyclohexane-tertbutanol were added to plasma samples with different propofol concentration. After being vibrated, the samples were centrifugated. The upper-clear solution was taken to determine the fluorescence intensity. The fluorescence detective excitation waves and emission waves were 265 nm and 310 nm respectively. RESULTS: Between the propofol concentrations and the fluorescence intensities, there was a good linear relationship in the range of 0.1-15.0 mg/L (r=0.9997). The average recovery was more than 95%. The relative standard deviation (RSD) of intra-day and inter-day assays were all less than 3%. CONCLUSION: The method is sample, accurate and sensitive for determining drug concentration of propofol in plasma.

关 键 词：异丙酚 血药浓度 荧光法 药代动力学 

分 类 号：R971.2[医药卫生—药品]