傅里叶变换红外光谱法研究HL-60细胞的分化过程  

Monitoring All-Trans-Retinoic Acid-Induced Differentiation of HL-60 Cells by Fourier-Transform Infrared Spectroscopy

作　　者：刘明杰[1] 王钊[1] 孙素琴[2] 吴庆余[1] 

机构地区：[1]清华大学生物科学与技术系,北京100084 [2]清华大学化学系,北京100084

出　　处：《光谱学与光谱分析》2005年第5期688-692,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(30271593);清华大学基础研究基金(JC2002046)资助项目

摘　　要：利用傅里叶变换红外光谱法(FTIR)研究了人白血病细胞HL60在全反式维甲酸(ATRA)作用下向粒细胞分化的过程。结果表明,分化后的HL60细胞FTIR图谱发生了显著变化,体现在与蛋白质、脂类、核酸和多糖等生物大分子相关的特征性谱带上。细胞内脂类物质烃链增长,含量增加。指纹图谱区域(900～1300cm-1)的谱带随着分化进程呈现规律性的变化。其中,核酸含量相对增加,并在1052和1153cm-1附近出现了新的谱峰,二阶导数谱进一步发现在1022cm-1处出现新峰,这说明蛋白糖基化,磷酸化以及核酸氢键加强作用在HL60细胞分化的过程中起着重要作用。通过计算一些图谱参数,并与硝基四唑氮蓝(NBT)还原实验相比较,证明红外图谱的变化与分化程度成正相关。This study reports a new in vitro analytical method, based on Fourier-transform infrared (FTIR) spectroscopy, to monitor the myeloid differentiation process in human myeloblast leukemia HL-60 cells induced by all-trans-retinoic acid (ATRA). The alteration of characteristic bands was identified in the differentiated cells, arising from proteins, lipids, carbohydrates and nucleic acids. Besides the changes in lipid content and plasma membrane fluidity, the most striking changes were observed in the region of nucleic acids and carbohydrates. The authors speculate that the glycosylation and phosphorylation of proteins and the hydrogen-bonding of nucleic acids were involved in differentiation. The spectral parameters were correlated with the differentiation index, as determined by NBT reduction assay. These results suggest that FTIR spectroscopy can be used to monitor the differentiation process of HL-60 cells.

关 键 词：FTIR HL-60细胞 分化 ATRA 

分 类 号：Q254[生物学—细胞生物学]