克百威与DNA之间作用的光谱学研究  

The Mechanism of Carbofuran Interacts with Calf Thymus DNA

作　　者：张立金[1] 闵顺耕[1] 李国学[2] 熊艳梅[1] 孙英[1] 

机构地区：[1]中国农业大学理学院,北京100094 [2]中国农业大学资源与环境学院,北京100094

出　　处：《光谱学与光谱分析》2005年第5期739-742,共4页Spectroscopy and Spectral Analysis

基　　金：农业部农药化学与应用技术重点开放实验室资金赞助;北京市重大自然科学基金(8000001)资助项目

摘　　要：利用吸收光谱法和荧光光谱法研究了克百威与ctDNA之间的相互作用。结果表明,ctDNA可使克百威的吸收光谱发生减色以及红移现象;ctDNA对克百威的荧光存在静态的荧光猝灭。两种光谱都支持克百威是以嵌插方式与ctDNA形成两者的加合物。溶剂以及离子强度对于两者加合作用存在不同的影响。Carbofuran is an insecticide used on a variety of corps. Acute and chronic occupational exposure of humans to carbofuran has been observed to cause cholinesterase inhibition, but little is known about the interaction of carbofuran with DNA. Using the technique of UV spectrum and fluorescence quenching respectively, the interaction between carbofuran and ct DNA was studied. The UV spectrum showed that ct DNA can lead to the hypochromic effect and red shift of the UV spectrum of carbofuran. The quenching process was proved to be the single static quenching and quenching constant decreases with temperature increasing. The basis of this specificity is intercalation of insecticide between base pairs to produce ct DNA-carbofuran adducts. Furthermore, ethanol can produce Franck-condon effect on the ct DNA-carbofuran adducts. At different sodium chloride concentrations, quenching constant had no significant change, which appeared that there was little electrostatic interaction between ct DNA and carbofuran and it was intercalation.

关 键 词：机理 克百威 ctDNA加合物 紫外 荧光猝灭 嵌插 

分 类 号：R931.6[医药卫生—生药学]