巯基葡聚糖凝胶分离富集微乳液增敏三甲氧基苯基荧光酮光度法测定痕量铜  

Spectrophotometric Determination of Trace Amount of Copper with Trimethoxyphenylfluorone in Microemulsion after Preconcentration and Separation by Sulphydryl Dextran Gel

作　　者：魏琴[1] 杜斌[1] 吴丹[1] 欧庆瑜[2] 

机构地区：[1]济南大学化学化工学院,山东济南250022 [2]中国科学院兰州化学物理研究所,甘肃兰州730000

出　　处：《光谱学与光谱分析》2005年第5期772-775,共4页Spectroscopy and Spectral Analysis

基　　金：山东省自然科学基金(Y2000B09);山东省计划项目(03C05)资助

摘　　要：研究在微乳液介质中,pH10.40时,铜与三甲氧基苯基荧光酮(TM PF)显色生成稳定的1∶2配合物,在552nm处摩尔吸光系数为1.46×105L·mol-1·cm-1,铜含量在0～0.6μg·mL-1范围内符合比耳定律,引入微乳液介质,显著改善了铜的显色条件,使体系灵敏度提高。采用吸附容量大,而机械性能好的巯基葡聚糖凝胶分离富集,消除了共存离子的干扰,降低了测定体系的检出限。测定了血样、尿样、水样和矿石样品中铜的测定,相对标准偏差低于4.8%,回收率为94.2%～106%。In this paper, the color reaction of copper with trimethoxyphenylfluorone (TM-PF) in microemulsion has been studied. Copper (II) reacts with TM-PF to form a stable 1: 2 complex at pH 10.40. Its apparent molar absorptivity is 1.46 X 10(5) L center dot mol(-1) cm(-1) at 552 nm. Beer's law is obeyed in the range of 0-0.6 mu g center dot mL(-1). Sulphydryl dextran gel (SDG), in which sulphydryl group has a big share, is a new type of metallic ion adsorbent. It presents the advantage of higher adsorption capacity, better reuse ability and higher mechanical stability. So the coexistent metal ions can be separated effectively and copper can be enriched by SDG, greatly improving the selectivity and sensibility of the system. The method was used to determine trace amount of copper in blood, urine, iron ore and water samples with satisfactory results, the relative standard deviation is less than 4.8%, and the recovery is 94.2%-106%.

关 键 词：三甲氧基苯基荧光酮 铜 微乳液 生物样品、水样和矿石 巯基葡聚糖凝胶 分离富集 光度法 

分 类 号：O657.3[理学—分析化学]