大鼠胃内伤害性刺激后延髓内脏带中Fos蛋白及OX_(42)的免疫组织化学观察  

OBSERVATION ON THE IMMUNOHISTOCHEMICAL LABELLING OF Fos PROTEIN AND OX_(42) ON THE MVZ IN RAT FOLLOWING STOMACHIC NOCICEPTION

作　　者：刘煜[1] 宿长军[2] 段丽[1] 曹荣[1] 饶志仁[1] 

机构地区：[1]第四军医大学神经科学研究所 [2]第四军医大学唐都医院神经内科,西安710032

出　　处：《解剖学报》2005年第3期237-240,共4页Acta Anatomica Sinica

基　　金：军队"十五"重点项目基金资助(01Z082)

摘　　要：目的研究大鼠延髓内脏带(MVZ)内小胶质细胞和神经元对福尔马林诱发胃伤害性刺激的可塑性反应及其相互关系。方法经细塑料管向胃内注入2.5%福尔马林2ml诱发胃伤害性刺激,动物存活0.5h、1h、2h和3h处死,延髓切片进行抗Fos蛋白(标记神经元)或抗OX42(标记小胶质细胞)单一或双重标记的ABC法染色。结果1.动物清醒后,在5～10min内出现躁动不安,持续1～2h;2.胃黏膜有局灶性出血和脱落;3.MVZ内观察到Fos阳性神经元和OX42阳性小胶质细胞,两者分布相似,显示明显的定位特点;4.双标记显示MVZ内Fos阳性神经元周围有密集的OX42阳性细胞;5.OX42阳性小胶质细胞的反应先逐渐升高(0.5～2h),后(3h)又降低,Fos阳性神经元则呈逐渐升高的趋势。结论MVZ的小胶质细胞可能与神经元一起参与内脏痛的调节。Objective To investigate the distribution relationship of microglia and neuron in rat medullary visceral zone(MVZ) after stomatic nociception. Methods After 2ml formalin(2.5%)injection in rat stomach by tiny plastic tube,Anti-Fos protein(labeling neuron)and anti-OX 42(labeling microglia)single and double immunohistochemical ABC method staining on model medulla oblongata sections in different time point(0.5h,1h,2h and 3h) were used. Results 1.After wakefulness,the animal models appear restlessness and agitation in 1-2h.2.We found hemorrhage and exfoliation in model's gastric mucosa.3.Fos positive neurons and OX 42 positive microglias were observed in MVZ,whose distribution was congruous and appeared clearly character of locations.4.The results of immunochemical diplex staining indicate that many Fos positive neurons were surrounded by intensive OX 42positive microglias.5.OX 42 positive microglia showed a tendency,which increased in 0.5-2h and decreased in 3h.Conclusion Neurons and microglia in MVZ may be involved in visceral nociception transfer and adjustment function.

关 键 词：神经元 小胶质细胞 免疫组织化学 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]