卵泡刺激素及其受体在大鼠胰腺的共定位观察  

OBSERVATION ON COLOCALIZATION OF FOLLICLE-STIMULATING HORMONE AND ITS RECEPTOR IN RAT PANCREAS

作　　者：初晨宇[1] 吕葆真[1] 孙岚[1] 黄威权[1] 王世鄂[1] 付建芳[1] 

机构地区：[1]第四军医大学组织学与胚胎学教研室,西安710032

出　　处：《解剖学报》2005年第3期303-305,共3页Acta Anatomica Sinica

摘　　要：目的观察卵泡刺激素(FSH)及其受体(FSHR)在大鼠胰腺中的共存关系,以了解FSH对胰腺的功能意义。方法应用免疫荧光组织化学双标记染色技术,在激光共聚焦扫描显微镜下观察染色结果。结果部分外分泌腺细胞、导管上皮细胞及部分胰岛细胞呈FSH反应阳性,呈强绿色荧光。小血管内皮细胞也呈FSH反应弱阳性,呈弱的绿色荧光。部分胰岛细胞呈FSHR反应阳性,呈强红色荧光。一些外分泌腺上皮细胞呈FSHR反应弱阳性,呈淡红色荧光。有一些胰岛细胞呈FSH和FSHR双阳性反应,呈黄色荧光或红、绿相间荧光。结论FSH及其受体均存在于大鼠胰腺中,胰岛中的部分细胞共同存在FSH及其受体,FSH可能通过旁分泌或自分泌的途径对胰腺的功能起调节作用。Objective To observate the colocalization of follicle_stimulating hormone(FSH)and its receptor(FSHR) in rat pancreas, supplying morphological evidence of functional significance of FSH in pancreas. Methods Double label immunofluorescence histochemical staining and confocal laser_scanning microscope were used in the experiment. Results The FSH positive products were located in some cells of exocrine portion and epithelial cells of gland duct, some islet cells and endothelial cells of blood vessel. These cells showed green fluorescence in cytoplasm. The FSHR positive products were located in some islet cells and some epithelial cells of exocrine gland. The positive products distributed in cytoplasm showing red fluorescence. Both FSH and FSHR positive products were co_located in some islet cells showing yellow or green and red fluorescence.Conclusion Both FSH and FSHR were presented in rat pancreas. The FSH and FSHR co_located in some islet cells. These results suggested that FSH may regulate the function of pancreas through paracrine and autocrine.;

关 键 词：卵泡刺激素 卵泡刺激素受体 胰腺 免疫荧光组织化学 激光共聚焦扫描显微镜 大鼠 

分 类 号：R333.3[医药卫生—人体生理学]