紫外分光光度法测定维乐生片含量  

Ultraviolet spectrophotometric assay for weilasheng tablet

作　　者：李献洲[1] 黎更豪[1] 

机构地区：[1]广东省江门市人民医院

出　　处：《中国医院药学杂志》1994年第6期267-269,共3页Chinese Journal of Hospital Pharmacy

摘　　要：采用紫外分光光度法测定维乐生片中维生素Ｂ１和维生素Ｂ６含量，以磷酸盐缓冲液（ｐＨ７．０）作溶媒，制成溶液在３２５ｎｍ波长处测得的Ａ值为维生素Ｂ６的吸收度，吸收系数（Ｅ）为３５０；在２６８ｎｍ波长处测得的Ａ值减去Ａ３２５×０．２３２后的差值为维生素Ｂ１的吸收度，吸收系数（Ｅ）为２４５。回收率维生素Ｂ６为１００．２％，ＲＳＤ＝０．３％；维生素Ｂ１为１００．９％，ＲＳＤ＝０．９％。维生素Ｂ１２含量测定按广东省标准法。he contents of vitamine B1 and B6 in the weilasheng tablet was determined by ultraviolet spectrophotometry.Phosphatebuffer(pH 7.0)was used as solvent. The absorbance of vitamme B6 was measured at 325 nm,its E1%1cm was 350,the ab-sorbance of vitamine B1 was the difference of A268 and 0.232 A325,theE1%1 cm was 245. The recovery of vitamine B6 was100.2%RSD=0.3%.and of vitamine B1,was 100.9%,RSD=0.9%.

关 键 词：维乐生片 分光光度法 含量 

分 类 号：R927.2[医药卫生—药学]