反相高效液相色谱法测定阿昔洛韦胶囊在人体内的药动学  

RP─HPLC determination of acyclovir and its pharmacokinetics in volunteers

作　　者：周梅华[1] 郭幼梅[1] 张称心 张莉[1] 杨香绥 李巧云[1] 韩群[1] 李端[1] 

机构地区：[1]上海医科大学药学院

出　　处：《中国医院药学杂志》1994年第10期437-438,共2页Chinese Journal of Hospital Pharmacy

摘　　要：本文建立了用反相高效液相色谱法测定阿昔洛韦血浓度的方法。流动相为４％甲醇水溶液，流速为２．０ｍｌ／ｍｉｎ，固定相为ＲＰ─Ｃ１８（５μｍ），检测波长２５４ｎｍ；以液━固萃取为前处理，扑热息痛为内标，阿昔洛韦与内标的保留时间分别为７．１０和１２．２７ｍｉｎ。本法最低检测限为３ｏｎ，ＲＳＤ＜５％。操作简便，适合于阿昔洛韦在体内药物动力学研究。n RP─HPLC method for determining serum level of acyclovir was established. Acyclovir extracted by Sep─Pak pretreat─ment cartridge could be detected at 254nm..An RP-C18 was used as the stationary phase and a solution composed of methanol:water(4:96V/V)as the mobile phase with a flow rate of 2.0ml/min.P-acetylaminophenol was used as an internal stan-dard.The retention time were 7.10and 12.27min respectively.The limit of detection was about 3 ng.The peak area ratio of the acyclovir and the interal standard against acyclovir concentration was linear with a correlation coefficient of 0.9998 over method is simple and precise and usable for pharmacokinetic studies in human beings.

关 键 词：阿昔洛韦 药物动力学 高效液相色谱 

分 类 号：R969.1[医药卫生—药理学]