缺氧性肺动脉高压大鼠肺组织中血小板源性生长因子和c－myc基因表达增强  

INCREASED EXPRESSION OF PLATELET-DERIVED GROWTH FACTOR AND c-myc ONCOGENE IN LUNGS OF HYPOXIC PULMONARY HYPERTENSIVE RATS

作　　者：韩梅[1] 蔡英年[1] 邓希贤[1] 

机构地区：[1]中国医学科学院基础医学研究所,河北医学院生化教研室

出　　处：《中国应用生理学杂志》1994年第4期293-296,共4页Chinese Journal of Applied Physiology

摘　　要：本研究分析了大鼠肺组织中血小板源性生长因子Ａ链、Ｂ链（ＰＤＧＦ－Ａ，ＰＤＧＦ－Ｂ）和ｃ－ｍｙｃ原癌基因ｍＲＮＡ在正常和缺氧时的含量变化。正常肺组织可表达１．７ｋｂ的ＰＤＧＦ－ＡｍＲＮＡ和３．５ｋｂ的ＰＤＧＦ－ＢｍＲＮＡ，还有少量２．２ｋｂｃ－ｍｙｃｍＲＮＡ。在缺氧过程中，ＰＤＧＦ－Ｂ链ｍＲＮＡ和ｃ－ｍｙｃｍＲＮＡ迅速增加，至缺氧１４ｄ时，分别为正常的３倍和５倍。而ＰＤＧＦ－ＡｍＲＮＡ在缺氧７ｄ时增高，而后又略有降低。结果表明：缺氧的肺组织局部生成的ＰＤＧＦ激活了ｃ－ｍｙｃ原癌基因，这对于缺氧性肺动脉高压的形成具有重要作用。The present study examined gene expression of platelet-derived growth factor(PDGF) andc-myc in the lung tissue of rats exposed to hypoxia.Normal lungs expressed PDGF-A chaintranscripts of 1.7 kb and PDGF-B chain transcripts of 3.5 kb. The c-myc transcript of 2.2 kb isalso expressed. After hypoxic exposure mRNA levels of the PDGF-B chain and the c-myc in-creased rapidly with 3-fold and 5-fold increase by day 14 respectively. PDGF-A chain mRNAincreased after hypoxic exposure for 7 days,and then declined. These results suggest thatactivation of autocrine and/or paracrine is important in proliferation mechanism of pulmonaryarterial smooth muscle cells in hypoxic pulmonary hypertensive rats.

关 键 词：缺氧 C-MYC原癌基因 肺动脉高压 肺 生长因子 

分 类 号：R543.202[医药卫生—心血管疾病]