重组人IL6／2融合蛋白对6．5Gy照射小鼠造血功能恢复的影响  

EFFECT OF RECOMBINANT hFPIL6/2 ON RECOVERY OF HEMOPOIESIS IN IRRADIATED MICE

作　　者：董波[1] 赵春华[1] 从玉文[1] 毛宁[1] 罗庆良[1] 邱丽玲[1] 毛秉智[1] 唐佩弦[1] 

机构地区：[1]北京放射医学研究所,北京基础医学研究所

出　　处：《中国应用生理学杂志》1994年第4期333-336,共4页Chinese Journal of Applied Physiology

摘　　要：观察了ｈＦＰＩＬ６／２对６．５Ｇｙγ线照射ＮＩＨ小鼠第１０天造血功能恢复的影响。结果表明：照射小鼠连续４ｄ给予ｈＦＰＩＬ６／２２５０μｇ·ｋｇ－１·ｄ－１，其脾重、ＣＦＵ－８、骨髓有核细胞数及ＣＭ－ＣＦＵ分别比对照组增加５９．０％、２７８．５％、５７．９％和１３８．２％，统计学处理均有显著差异；对此四项指标的改善也明显优于２５μｇ组。另外，２５０μｇ剂量组小鼠外周血象３０ｄ的动态观察结果表明，ｈＦＰＩＬ６／２不但能明显提高红细胞和血红蛋白的最低值，而且能使血小板的恢复提前。提示ｈＦＰＩＬ６／２在促进血小板生成和促进红系造血方面可能具有良好的应用前景。The effect of hFPIL6/2 on recovery of hemopoiesis in mice exposed to 6.5Gy of gammairradiation was observed.NIHmice were subcutaneously administered either saline orhFPIL6/2 b.i.d at doses of 25 and 250μg·kg-1·d-1 for 4 consecutive days after irradiation.On day 10 postirradiation, the spleen weight,CFU-s,bone marrow nucleated cell andGM-CFU at the dose of 250μg increased by 59.0%, 278.5%, 57.9 % and 138.2% as comparedwith the control group respectively, with significant difference(P<0.05 or P < 0.01).LowhFPIL6/2 dose groups(25μg· kg-1·d-1) showed a promotive effect on recovery.In addition,the levels of RBC,Hb,WBC and platelet in peripheral blood were also raised by high dose ofhFPIL6 / 2(250μg).The results indicate that hFPIL6 / 2 has beneficial effects on hemopoietic re-covery in the sublethally irradiated mice.

关 键 词：融合蛋白 Γ辐射 白细胞介素 放射保护药 

分 类 号：R979.6[医药卫生—药品]