淡紫拟青霉诱变菌株的RAPD分析  

RAPD Analysis of 16 Isolates of Paecilomyces lilacinus IPC

作　　者：张延新[1] 刘开启[2] 夏振远[3] 李天飞 

机构地区：[1]山东农业大学植物保护学院,泰安271018 [2]仲恺农业技术学院植物保护系,广州510225 [3]云南烟草科学研究所,玉溪653100 [4]玉溪红塔集团,玉溪653100

出　　处：《中国生物防治》2005年第2期109-112,共4页Chinese Journal of Biological Control

基　　金：广东省自然基金(021923);云南省"十五"攻关项目(2001NG08);国家烟草专卖局科技项目(110200101010)

摘　　要：选用6个随机引物对淡紫拟青霉IPC菌株及其15株突变菌株的全基因组DNA进行随机多态性扩增,共扩增出114条250～2500bp的DNA片段,多态检测率为83.3%。利用UPG MA方法对扩增的DNA片段聚类分析,结果表明:供试菌株具有丰富的遗传多样性,应用RAPD技术可以快速准确地鉴定菌株是否突变及突变程度;参照突变菌株表型特性上的改变,该方法可以作为确定其遗传性上是否发生分化的依据。The genomic DNA of IPC and its 15 mutated isolates was put through RAPD with 6 arbitrary primers. 114 DNA fragments ranged from 250 to 2500bp was amplified. Polymorphism detection rate was 83.3%. Cluster analysis of the RAPD profiles by using UPGMA displayed that the studied isolates have genetic polymorphism. The RAPD can detect the mutation and its degree quickly and accurately. It also provided proof for the genetic changes of the mutated isolates which show changes of phenotypic characteristics.

关 键 词：淡紫拟青霉 遗传多样性 RAPD 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]