大鼠肾移植亚临床急性排斥反应中T淋巴细胞亚群的变化及其意义  

Changes and significance of T lymphocyte subsets in subclinical acute rejection of rat renal transplantation

作　　者：韩志军[1] 祝青国[1] 仇宇[1] 赵亚昆[1] 于永[1] 

机构地区：[1]哈尔滨医科大学附属第二医院泌尿外科,黑龙江哈尔滨150086

出　　处：《中国现代医学杂志》2005年第8期1198-1199,1203,共3页China Journal of Modern Medicine

摘　　要：目的建立大鼠异体肾移植模型,研究肾移植中T细胞亚群的变化及其与亚临床急性排斥反应的关系。方法实验分为5组,第1～4组为异品系移植组(SDtoWistar);第5组为同品系移植组(WistartoWistar)作为对照组,观察第1、2、3、5组移植术后排斥反应;用免疫荧光染色流式细胞仪检测第4、5组手术前后T细胞亚群。结果异品系移植组(SD→Wistar)大鼠肾移植亚临床急性排斥反应于术后3、4d出现;CD4/CD8比值于术后第2天显著增高,同品系移植组(WistartoWistar)术后,CD4/CD8较术前无明显变化,未出现排斥反应。结论肾移植术后T细胞亚群中CD4/CD8比值的变化比病理改变提前出现1、2d,可作为监测异体肾移植亚临床急性排斥反应的免疫学指标。[Objective]To explore the relation between the subclinical acute rejection and T lymphocyte subsets through model of renal transplantation in rats. There were five groups. In 1～4 groups, SD to Wistar, renal transplantation were performed. In the 5th group, Wistar to Wistar. In 1st～3rd groups and the 5th group, the time of subclinical acute rejection of renal transplantation were investigated. In the 4th and 5th group, T lymphocyte subsets were assayed using immunofluorence stain and flow cytometry before and after operation, and relationship between the changes in T lymphocyte subsets and the rejection time was observed. In allografts, subclinical acute rejection occurred on the 3rd or 4th day after operation. In the 4th group, the ratio of CD4/ CD8 was obviously increased on the 2nd day after operation. In the 5th group, there was no significant difference in the ratio CD4/ CD8 before and after operation, and no rejection occurred. [Conclusion] The changes of CD4/ CD8 were about 1～2 days earlier than pathological changes in rat allografts, so it can act as an immunological indicator to monitor subclinical acute rejection of rat allografts.

关 键 词：大鼠 肾移植 亚临床急性排斥反应 T细胞 

分 类 号：R65[医药卫生—外科学]