实时心肌造影在基因转染细胞移植治疗慢性缺血心肌的研究应用  

Evaluation of Chronic Ischemic Myocardium before and after Transplantation of Bone Marrow Stromal Cell Transferred by Angiogenin Gene by Real-time Myocardial Contrast Echocardiography

作　　者：赵宝珍[1] 徐静[1] 王韦[1] 龚德军[2] 崔勇[2] 贾宝成[2] 周桂花[1] 王虹[1] 

机构地区：[1]上海第二军医大学长海医院超声科 [2]长海医院胸心外科实验室,上海200433

出　　处：《声学技术》2004年第F11期12-16,共5页Technical Acoustics

摘　　要：目的:用实时心肌声学造影(RTMCE)研究基因转染骨髓基质干细胞移植治疗慢性缺血心肌的效果。方法:实验动物(猪)分4组,建立慢性心肌缺血模型4周后,组Ⅰ注射编码血管生长素(ANG)基因的腺病毒转染的自体骨髓基质干细胞,组Ⅱ注射编码ANG基因的腺病毒液,组Ⅲ注射单纯自体骨髓基质干细胞;组Ⅳ(对照组)注射空腺病毒磷酸盐缓冲液。再4周后取心肌组织进行病理学检查。分别于正常状态、慢性缺血心肌模型建立4周和注射后4周开胸时进行RTMCE,选取左室乳头肌水平短轴切面,用闪烁显像观察局部心肌灌注显影情况。实时动态图像存入磁光盘,然后用MCE定量分析软件(CUSQ1. 4)分析各阶段心肌灌注图像。结果RTMCE较满意显示了猪慢性缺血心肌基因治疗前后心肌运动和血流灌注状况。经定量软件对闪烁显像心肌再灌注分析,发现慢性缺血心肌模型各组左室侧后壁A值和β值均较正常心肌明显降低(P<0. 01),组间比较无明显差异(P>0. 05);注射4周后,组Ⅰ、组Ⅱ、组Ⅲ的A值和β值均增高,其中组Ⅰ增高最显著(P<0. 01),组Ⅱ和组Ⅲ较对照组亦明显增加(P<0. 05),对照组的A值和β值与缺血心肌无明显变化(P>0. 05)。结论RTMCE及其定量分析软件可对不同血供状态下的心肌灌注进行分析,评价基因治疗慢性缺血心肌的效果。Objective To investigate the value of real-time MCE(RTMCE)in detecting chronic ischemic myocardium before and after transplantation of bone marrow stromal cells (BMSCs) combined with angiogenic gene transfer. Methods Four weeks after chronic ischemic myocardium models were set up, groupⅠ-Ⅳ were intramyocardially administrated by BMSCs transferred by Adenovirus mediated Angiogenin gene(Ad·ANG), Ad·ANG, BMSCs and phosphate buffer(PB) respectively. Four weeks later, Pathological investigation of their hearts were performed. The RTMCE including flash imagining of open-chest porcine lateral-posterior wall at normal state, 4 weeks after Ameroid placement and 4 weeks after administration were performed. Quantitation analysis by CUSQ software was also performed. Results The real-time MCE can show myocardial perfusion changes of every group radiantly. Quantitation analysis show that A and β are all decreased 4 weeks after Ameroid placement and increased 4 weeks after administration. The increasing of A and β in groupⅠis most obvious(P<0.01). The increasing of groupⅡand Ⅲ are also more obvious than group Ⅳ(P<0.01). Conclusions RTMCE can show and analysis quantificationally different myocardial perfusion, and evaluate different gene therapy methods for chronic ischemic myocardium.

关 键 词：心肌声学造影 心肌缺血 骨髓细胞 基因治疗 

分 类 号：TB559[理学—物理]