三维超声心动图评价基因转染骨髓干细胞移植治疗慢性缺血心肌价值  

Assessment of Chronic Ischemic Myocardium before and after Transplantation of Bone Marrow Stromal Cell Transferred by Angiogenin Gene using Three-dimensional Echocardiography

作　　者：徐静[1] 赵宝珍[1] 王韦[1] 黄盛东[2] 贾宝成[2] 龚德军[2] 崔勇[2] 周桂花[1] 王虹[1] 

机构地区：[1]上海第二军医大学长海医院超声科 [2]长海医院胸心外科实验室,上海200433

出　　处：《声学技术》2004年第F11期40-44,共5页Technical Acoustics

摘　　要：目的：探讨动态三维超声心动图(DTDE)评价基因转染自体骨髓基质干细胞移植治疗慢性缺血心肌的价值。方法：在猪冠脉左旋支放置Ameroid环建立慢性心肌缺血模型；4周后分组注射编码血管生长素(ANG)基因的腺病毒转染的自体骨髓基质干细胞(组Ⅰ)、编码ANG基因的腺病毒液(组Ⅱ)、单纯自体骨髓基质干细胞(组Ⅲ)和空腺病毒磷酸盐缓冲液(组Ⅳ、对照组)；再4周后行病理学检查：应用脱机DTDE重建和分析技术，观测正常、置环后4周及注射后4周左室形状、容积和收缩功能的变化。结果：慢性缺血心肌左室舒张和收缩末期容积(LVEDV、LVESV)增大，射血分数(LVEF)降低(P<0．01)。注射后，对照组LVEDV、LVESV较其它各组明显增大(P<0．05)，射血分数(LVEF)明显降低(P<0．05)，与缺血时无明显差异(P>0．05)。组Ⅰ～Ⅲ较慢性缺血时LV-EDV和LVESV均缩小(P<0．05)，LVEF均增大(P<0．05)，各治疗组比较，左室重构现象均不同程度地减轻，组Ⅰ较其它组，左室容积减小及收缩功能改善最为显著。组Ⅱ和Ⅲ问测值比较无统计学差异。结论：DTDE对评价基因转染自体骨髓基质干细胞移植治疗慢性缺血性心脏病具有重要价值。Objective: To investigate the value of Dynamic Three-dimensional echocardiography (DTDE) in detecting chronic ischemic myocardium before and after transplantation of bone marrow stromal cells (BMSCs) combined with angiogenic gene transfer. Methods: Four weeks after chronic ischemic myocardium models were set up, groupⅠ-Ⅳ were intramyocardially administrated by BMSCs transferred by Adenovirus mediated Angiogenin gene(Ad·ANG), Ad·ANG, BMSCs and phosphate buffer(PB) respectively. Four weeks later, Pathological investigation of their hearts was performed. DTDE was performed at normal state, 4 weeks after Ameroid placement and 4 weeks after administration respectively. Results: The end-diastolic and end-systolic volume of left ventricle (LVEDV、LVESV) are all increased and the ejection fraction (LVEF) all decreased 4w after Ameroid placement (P<0.01). After administration, group Ⅳ remained increased volumes and decreased LVEF compared with that before administration and that of other administrated groups (P<0.05). The greatest increase of LVEDV、LVESV and greatest decrease of LVEF were found in Group Ⅰ in all administrated groups. Conclusions: DTDE is a feasible approach to assess LV volumes and systolic function changes in chronic ischemic myocardium before and after gene transfer and cell transplantation therapy.

关 键 词：动态三维超声心动图 心肌缺血 骨髓细胞 基因治疗 

分 类 号：TB559[理学—物理]