耳蜗半薄切片细胞凋亡检测  

Detection of apoptosis in semithin sections of the cochlea

作　　者：郭维 杨仕明 胡吟燕 杨伟炎 

机构地区：[1]解放军总医耳鼻咽喉科研究所,北京100853

出　　处：《中华耳科学杂志》2005年第2期126-127,共2页Chinese Journal of Otology

基　　金：国家自然科学基金(30000189);军队"十五"科研基金(01Q050);北京市自然科学基金(7042061)资助

摘　　要：目的尝试在耳蜗半薄切片上用TUNEL方法检测耳蜗组织的细胞凋亡。方法基因敲除Smad5小鼠耳蜗,分别作常规石蜡切片和环氧树脂包埋的半薄切片,用TUNEL方法检测耳蜗组织的细胞凋亡。结果在半薄切片上Smad5基因敲除小鼠,耳蜗内有大量的凋亡细胞产生,主要集中在螺旋神经节细胞、血管纹上的细胞,以及基底膜上的间皮细胞等,而且能够更清楚的显示凋亡的毛细胞。而在石蜡切片上只在螺旋神经节细胞、血管纹上的细胞,以及基底膜上的间皮细胞检出凋亡细胞,但是毛细胞没有凋亡细胞的检出。结论用TUNEL方法检测耳蜗组织的细胞凋亡半薄切片明显好于石蜡切片。Objective To detect the cell apoptosis in semithin sections of cochlea using the TUNEL method . Methods The semithin sections and paraffin sections were made from cochlea of mice with Smad5 knocked out, and then apoptotic cell were detected with the TUNEL method. Results Apoptotic cells in spiral ganglion, stria vascularis and mesothelial cells of basement membrane can be checked by both semithin and paraffin sections of cochlea. While apoptotic hair cells can be detected only in semithin but not paraffin sections. Conclusion The method of semithin sections was better than paraffin sections to detect the cell apoptosis of cochlea using the TUNEL method.

关 键 词：半薄切片 凋亡检测 TUNEL方法 螺旋神经节细胞 SMAD5 细胞凋亡 耳蜗组织 环氧树脂包埋 常规石蜡切片 基因敲除小鼠 凋亡细胞 间皮细胞 血管纹 毛细胞 膜上 基底 检出 

分 类 号：R446[医药卫生—诊断学] R711.71[医药卫生—临床医学]