两种不同细菌所致急性中耳炎对内耳损伤的差异  

The difference of inner ear ultrastructure in acute otitis media induced by different virulent bacteria

作　　者：苏钰[1] 马宏敏[1] 孙景豫[1] 盛宏申[1] 刘顺利[1] 黄维国[1] 邱建华[1] 

机构地区：[1]西安第四军医大学西京医院全军耳鼻咽喉专科中心,西安710033

出　　处：《中华耳科学杂志》2005年第2期128-131,共4页Chinese Journal of Otology

基　　金：国家自然科学基金资助项目(30371528)

摘　　要：目的研究两种毒力不同的细菌导致的急性中耳炎对内耳超微结构损伤的差异。方法豚鼠麻醉后,一组在无菌条件下于右耳经鼓膜注射1×10·8L-1的绿脓杆菌液100μL,左耳作正常对照;另一组同法注射金葡菌液。三天后常规处理,透射、扫描电镜观察。结果造模后三天,绿脓杆菌感染组及金葡菌感染组基底膜、血管纹均可见细胞损伤、细胞器变性,绿脓杆菌感染组内耳超微结构的改变明显重于金葡菌感染组。结论急性中耳炎早期即可有内耳的形态学改变,绿脓杆菌引起的急性化脓性中耳炎可能会导致严重感音神经性聋。Objective To investigate the difference of inner ear ultrastructure in acute otitis media induced by different virulent bacteria. Methods Sixteen guinea pigs were randomly divided into two groups. The right middle ears were inoculated with 0.1ml 1×108/ml staphyloccoccus aureus or pseudomonas aeruginosa respectively. The left ear serves as control. On the 3rd day after infection, alteration of the cochlea was observed by scaning and transmisson electron microscopy. Results On the 3rd day after infection, the damage of cells and degeneration of organelles could be observed in both groups of staphyloccoccus aureus and pseudomonas aeruginosa. The alterations of ultrastructure in the pseudomonas aeruginosa-infected group are more apparent than those in the staphyloccoccus aureus-infected group. Conclusion There are morphological changes of the inner ear in early acute otitis media. The acute otitis media infected by pseudomonas aeruginosa may induce serious sensorineural hearing loss.

关 键 词：急性中耳炎 内耳损伤 细菌 急性化脓性中耳炎 绿脓杆菌感染 金葡菌感染 扫描电镜观察 感音神经性聋 形态学改变 结构损伤 正常对照 常规处理 细胞损伤 超微结构 麻醉后 无菌条 基底膜 血管纹 细胞器 菌液 注射 

分 类 号：R764.21[医药卫生—耳鼻咽喉科] R764.83[医药卫生—临床医学]