骨髓间充质干细胞复合去抗原松质骨修复骨缺损的实验研究  

Experiment Study Of MSCs Culture And Rats Segmental Radial Defects Repaired With MSCs/BCB Composite Graft

作　　者：曹豫江[1] 张德文[1] 陈致远[2] 刘官信[3] 

机构地区：[1]重庆医科大学附属儿童医院骨科,重庆400014 [2]重庆医科大学附属儿童医院外科教研室,重庆400014 [3]重庆医科大学附属儿童医院儿科研究所,重庆400014

出　　处：《生物骨科材料与临床研究》2004年第3期5-9,共5页Orthopaedic Biomechanics Materials and Clinical Study

摘　　要：目的探讨骨髓间充质干细胞离体培养并与去抗原牛松质骨复合修复节段性骨缺损的效果。方法Wistar大鼠骨髓间充质干细胞(MSCs)与去抗原牛松质骨(BCB)制成MSCs/BCB复合物;采用同种异体大鼠桡骨干5mm节段性骨缺损动物模型,通过X线放射学、组织学、生物力学检测对比研究骨缺损修复情况。结果术后2、4、8、14、18周实验组与实验对照组经放射学检查评价新骨生成有显著性差异。术后组织学检查新骨生成速度、生成量均有显著性差异。18周经生物力学实验检测,实验组与实验对照组新骨生成有显著性差异,实验组标本与正常基本一致。空白对照组各时间点均无新骨形成,最后缺损由纤维组织充填。结论大鼠骨髓间充质干细胞(MSCs)是骨组织工程中适宜的种子细胞。去抗原牛松质骨是可以选择的细胞载体。MSCs/BCB复合物植入修复骨缺损效果明显优于单纯BCB植入。Objective To discuss rat bone marrow mesenchymal stem cells(MSCs)'culture in vitro and the curative effect of repairing segmental bone defects in rats with the composite grafts of MSCs and anti-xtracted bovine cancellous bone(BCB).Method An bone defect (5mm in length) was created at two radii in each rat. The MSCs were cultured in combination with BCB. The curative effect was evaluated by radiographic examination, histologic analysis, biomechanical test after surgery. Result Roentgen graphically, The bone defects that had been treated with grafts exhibited new bone formation, increasing with time. But the result of experimental groups were apparently superior to the control group's at 2, 4, 8, 14, 18 week after operation. The rate and quality of new bone formation was significantly different in the experimental and the control groups on histologic analysis. The mechanical strength of composite grafts reached almost to normal level at 18 week. The loads and bendingstresses of experimental groups were remarkably higher than those of the control groups. The defects in blank groups, no formation of new bone were found after operation and were finally repaired only by fibrous tissue. Conclusion MSCs are ideal seeded cells in bone tissue engineering. BCB can be used as a alternative carrier for seeded cells. These studies demonstrate that the composite grafts more quickly promote the new bone formation.

关 键 词：骨髓间充质干细胞 去抗原牛松质骨 细胞培养 骨移植 骨缺损 

分 类 号：R687.3[医药卫生—骨科学]