大鼠骨髓间充质干细胞对同种异体骨髓移植造血重建的影响  

Effect of Rat Mesenchymal Stem Cells on Hematopoietic Reconstitution after Allogeneic Co-transplantation with Bone Marrow

作　　者：雷俊霞[1] 朱美玲 郭振宇[3] 赵东长[4] 余伟华[1] 温冠媚[5] 张秀明[1] 李艳[1] 项鹏[6] 李树浓[1] 

机构地区：[1]中山大学中山医学院病理生理教研室,广州510080 [2]深圳宝安区中心血站,深圳518101 [3]中山大学附属第五医院器官移植科,珠海519000 [4]广东省妇幼保健院儿科,广州510010 [5]广西医科大学病理生理教研室,南宁530021 [6]中山大学干细胞中心,广州510080

出　　处：《中国实验血液学杂志》2005年第3期397-403,共7页Journal of Experimental Hematology

基　　金：国家自然科学基金(编号30100188);广州市科技计划项目(编号:2002U13E0011)资助

摘　　要：为了探讨大鼠骨髓间充质干细胞(mesenchymalstemcell,MSC)对同种异体骨髓移植造血重建的影响,建立大鼠同种异体骨髓移植模型,通过外周血像检测、病理分析和流式细胞术检测综合评价MSC对骨髓移植(bonemarrowtransplantation,BMT)的积极作用。结果表明:①共移植后30天,MSC可在致死量照射的受者存活,并被发现受者胸腺、脾脏和骨髓;②MSC可促进BMT后造血重建,促进外周血白细胞、淋巴细胞和血小板的明显恢复;促进骨髓细胞数恢复及B淋巴系、巨核系分化增强;有利骨髓组织学的明显恢复发善。结论:大鼠MSC与骨髓共移植可在受体的造血器官长期存在,MSC可明显促进同种异体骨髓移植后造血重建。To investigate effects of rat bone marrow mesenchymal stem cells (rBMMSC) on hematopoiesis after allo-hematopoietic stem cell transplantation(HSCT), allogeneic BMT model from Fischer 344 rats (RT-1Al) to Wistar rats(RT-1Au) was established; effects of MSCs on hematopoietic reconstitution were studied by survival rate, peripheral blood counts, histological analysis and FACS at day 30 after transplantation. The results showed that (1) MSCs from donor Fisher344 could survive in recipient irradiated by lethal dose and could be found in the thymus, spleen and bone marrow of the recipient at 30 days after cotransplantation with BM by measuring EGFP gene. (2) Cotransplanation of MSCs and BM improved hematopoietic reconstitution. Lymphocyte and platelet counts of peripheral blood in cotransplantation group were higher than those in the control group. Active hematopoiesis and increase of bone marrow nucleated cells were observed in cotransplantation group. MSCs significantly enhanced hematopoiesis of B lymphocyte and megakaryocytopoietic lineages by FACS analysis. It is concluded that (1) MSCs of Fisher344 can be found in the thymus, spleen, bone marrow of the recipients at 30 days after cotransplantion by measuring EGFP gene. (2) hematopoietic reconstitution is significantly enhanced by MSCs cotransplanted with BM.

关 键 词：骨髓 间质干细胞 同种异体骨髓移植 造血重建 GVHD 

分 类 号：R457.7[医药卫生—治疗学]