化学修饰物甲氧基聚乙二醇对异基因骨髓移植影响的研究  

Study on Bone Marrow Transplantation Camouflaged with Methoxy Polyethylene Glycol

作　　者：龙卉[1] 唐锁勤[1] 张晓飞[1] 

机构地区：[1]解放军总医院小儿内科,北京100853

出　　处：《中国实验血液学杂志》2005年第3期408-411,共4页Journal of Experimental Hematology

摘　　要：为了探讨经甲氧基聚乙二醇(mPEG)修饰的骨髓移植物对异基因移植并发症———移植物抗宿主病(GVHD)的影响,将BALB/cH2d小鼠经60Coγ线8.0Gy照射后随机分3组。A组经尾静脉注射RPMI1640,B组注射615H2k小鼠的骨髓及脾细胞混合悬液,C组注射等量的mPEG修饰后的骨髓及脾细胞混悬液。通过小鼠外观的观察、存活率和存活时间的测定及组织病理检查研究GVHD程度,通过染色体检查研究植入情况。研究结果表明:B组出现脱毛等表现较C组早,程度重,重症病例多(P≤0.05)。B组死亡小鼠30天存活率20%,平均存活时间为12.1±2.4天,C组为50%,平均存活时间为16.1±2.9天(P≤0.05);B组出现Ⅲ度GVHD比例为59%,C组为20%(P=0.050),B组和C组的异基因骨髓成功植入。结论:mPEG修饰的骨髓移植物可减轻GVHD程度,有望延长生存时间,提高生存率。To explore the effect of bone marrow camouflaged with methoxy polyethylene glycol (mPEG) on allogeneic bone marrow transplantation, 60 BALB/c H-2d mice were randomly divided into 3 groups after irradiation by 8.0 Gy of 60Co γ ray. A group was given RPMI 1640 0.5 ml in tail vein. B group was infused with the bone marrow cells(1×107) mixed with the spleen cells(1×107)of donor 615 H-2k mice. C group was transplanted with same dose cells, which were camouflaged with mPEG before infusion. Severity GVHD was determined by total manifestation of mice, survival rate, survival time and histo-pathological microscopy, and engraftment of allogeneic bone marrow was evaluated by chromosome examination. The results showed that 75% mice in B group had severe adverse manifestations, such as hunched posture, diarrhea and loss of hair. Occurrence of the same adverse manifestations in C group was 35% and significantly lower than that in B group(P≤0.05). The mean survival time and survival rate of C group were 16.1 days and 50% respectively, which were higher than those of B group (12.1 days and 20%). White blood cells in peripheral blood of C group mice recovered more quickly than that in B group mice. The number of GVHD gradⅢ in B group was 59%, whereas in C group was 20% (χ2=3.844,P=0.050); allogeneic bone marrow was successfully engrafted in B and C groups. In conclusion, GVHD of allogeneic bone marrow transplantation can be alleviated by bone marrow cells camouflaged with mPEG, and such transplantation may be able to prolong survival time and increase survival rate.

关 键 词：异基因骨髓移植 移植物抗宿主病 甲氧基聚乙二醇 

分 类 号：R457.7[医药卫生—治疗学]