血小板活化因子参与血小板-中性粒细胞活化的研究  

Activation of Platelet-Neutrophil Mediated by Platelet-Activating Factor

作　　者：郭农建[1] 常亚丽[1] 肖东杰[1] 黄平[1] 

机构地区：[1]山东大学临床医学院,济南市中心医院

出　　处：《中国实验血液学杂志》2005年第3期447-451,共5页Journal of Experimental Hematology

摘　　要：本研究的目的是探讨全血中血小板活化因子(plateletactivatingfactor,PAF)参与血小板中性粒细胞相互作用的病理生理机制,为PAF受体拮抗剂的临床应用提供理论依据。将PAF受体拮抗剂银杏内酯B(ginkgolidesB,GBBN52021)加入全血中,以阻断PAF对血小板中性粒细胞的活化作用;将已知的PAF及二磷酸腺苷(ADP)分别加入全血中,用流式细胞术(FCM)检测血小板CD62P的表达;将已知的PAF及ADP分别加入全血中,用FCM检测中性粒细胞细胞CD11b的表达;将PAF及ADP分别加入全血中,用FCM检测血小板白细胞聚集物(PLA),即PLA/L包括百分率及CD42b中位荧光浓度(MFI)。结果表明:PAF及ADP均增加CD62P、CD11b、PLA的表达;PAF受体拮抗剂(银杏内酯B)明显抑制PAF诱导的CD62P、CD11b的表达及PLA的形成,但不能完全抑制血小板、中性粒细胞的活化及血小板白细胞之间的相互作用;银杏内酯B也抑制ADP诱导的CD62P、CD11b的表达,但不能完全抑制血小板、中性粒细胞活化;银杏内酯B对ADP诱导的血小板白细胞的聚集无明显抑制作用。结论:血小板中性粒细胞的相互作用机制复杂,有多种细胞因子、化学介质参与其中,有多种配基受体介导血小板中性粒细胞的交互作用。PAF受体拮抗剂—银杏内酯B有明显拮抗PAF的作用,在防治血栓形成与炎症过程中可能具有广泛的应用?To investigate the pathophysiological mechanisms for platelet-neutrophils cross talk mediated by platelet-activating factor (PAF) and to lay a foundation for clinical application, ginkgolides B (GB), a PAF receptor antagonist, was added in the whole blood to block the effects of PAF on activation of platelet-neutrophil; PAF and ADP were respectively added in the whole blood to monitor the expression of CD62P on platelet by flow cytometry; PAF and ADP were added in the whole blood to monitor the expression of CD11b on neutrophil by flow cytometry; PAF and ADP were added in the whole blood to monitor the platelet-leucocyte aggregates (PLA) which were PLA in the total leucocyte population (PLA/L) and the mean fluorescence intensity (MFI) of CD42b.Outcomes were analyzed by t-test ,and the differences were statistically significant(P <0.05). The results showed that the expression of CD62P on platelats, the expression of CD11b on neutrophils and PLA formation were all increased by PAF and ADP; the PAF receptor antagonists (GB) could obviously inhibit the expression of CD62P, CD11b and PLA formation induced by PAF, but could not completely inhibit the activation of platelet and neutrophil, and the platelet-neutrophil cross talk; GB could inhibit the expression of CD62P and CD11b induced by ADP, but could not conpletely inhibit the activation of platelet and neutrophli; GB could not obviously inhibit the platelet-leucocyte aggregates mediated by ADP. It is concluded that the multiligand-receptor systems involved in PLA formation and platelet-netrophils cross talk seem to be regulated by complex mechanisms; the PAF receptor antagonists (GB) obviously inhibit the effect of PAF, and may be widely utilized in the therapy of thrombosis and inflammation.

关 键 词：血小板活化因子 血小板 中性粒细胞 银杏内酯B 

分 类 号：R331.143[医药卫生—人体生理学] R364.15[医药卫生—基础医学]