同种与异种胎胸腺混合移植诱导供者皮肤移植免疫耐受  

Induction of immune tolerance to donor skin grafts by mixed transplantion of allogeneic and xenogeneic fetal thymus

作　　者：王锡华[1] 吴军[1] 解志杰[1] 袁军[1] 贺伟峰[1] 陈希炜[1] 易绍萱[1] 

机构地区：[1]解放军第三军医大学西南医院烧伤研究所,重庆市400038

出　　处：《中国临床康复》2005年第18期77-79,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学青年基金项目(30200261)~~

摘　　要：目的:探讨同种异种胎胸腺混合移植达到供者皮肤移植的耐受。方法:实验于2003-03/12在第三军医大学创伤、烧伤、复合伤实验室完成。将F344大鼠胎胸腺和C57BL/6小鼠胎胸腺混合移植到BALB/C裸小鼠的双肾包膜下4个月后,用流式细胞仪技术检测裸小鼠外周血中CD3+和CD4+T细胞,用单向混合淋巴细胞培养了解T细胞对ConA以及同种异种淋巴细胞的反应性,异基因皮肤移植观察皮肤移植物存活期。结果:①混合移植胎胸腺4个月后,受者裸小鼠外周血中CD3+和CD4+T细胞占总白细胞的25.9%,正常裸小鼠外周血中CD3+和CD4+量极少(2.43%)。②受者T细胞对ConA以及无关的SD鼠异种淋巴细胞的反应性强(刺激指数分别为23.23与12.75),而对供者F344及C57BL/6淋巴细胞不发生增殖反应(刺激指数分别为1.12与0.98)。③受者对供者F344及C57BL/6鼠皮肤移植物86d不发生排斥,而对无关的SD鼠皮肤移植物15d即发生排斥。结论:受体对胸腺供体来源的F344和C57BL/6皮肤及宿主本身的BALB/C皮肤移植耐受,而对无关的SD鼠皮肤移植物较短时间内即发生了排斥,并且抗DNA自身抗体水平与正常鼠相似。表明同种和异种胸腺混合移植后受体可重建有免疫功能的T淋巴细胞,并能诱导供者特异性免疫耐受的发生。AIM:To investigate the immune tolerance to donor skin graft resulting from th e mixed transplantion of allogeneic and xenogeneic fetal thymus. METHODS:The experiment was conducted in the Laboratory for Trauma,Burns and C ombined Injury in the Third Military Medical University from March to December 2 003.Four months after the mixed thymuses of fetal F344 rat and C57BL/6 mouse wer e transplanted into BALB/C nude mice under the double kidney capsules,the conten ts of CD3+ and CD4+ T lymphocytes in peripheral blood in the recipients were detected by flow cytometry,and T cell response to stimulation of ConA mitogens and allogeneic or xenogeneic lymphocytes was measured by single- phase mixed ly mphocyte reaction(MLR),and the survival time of skin graft was observed by xenog eneic skin grafting. RESULTS:① At 4 months after mixed fetal thymus transplantation,the CD3+ an d CD4+ T cells in the peripheral cells of recipient mice was 25.9% of the who le white blood cells,but those of normal nude mice were relatively fewer(2.43% ).② In MLR,T cells from recipients showed a strong response to either the stimu lation of ConA mitogens or SD rat lymphocytes(stimulation index was 23.23 and 12 .75 respectively),and no proliferative response to the lymphocytes of donor F344 rat and C57BL/6 mouse(stimulation index was 1.12 and 0.98 respectively).③ The recipients did not reject the donor F344 or C57BL/6 skin grafts within 86 days,b ut rejection was found in the SD rat recipients at 15 days after transplantation . CONCLUSION:Recipients were tolerant to the donor F344 or C57BL/6 skin grafts and host BALB/C skin grafts,but rejected the SD rat skin grafts in a short time, and the level of anti- DNA autoantibody was similar to that of normal rats.Afte r the mixed F344 rat and C57BL/6 mouse fetal thymus transplanted into nude mice can efficiently reconstitute the T cells with immune function,and induce donor- specific immune tolerance.

关 键 词：胸腺移植 免疫耐受 皮肤移植 

分 类 号：R318[医药卫生—生物医学工程]