先天性马蹄内翻足细胞外基质的变化(英文)  

Changes of extracellular matrix of congenital clubfoot

作　　者：赵东风[1] 岳勇[1] 杨柏[1] 葛杰[1] 汤志宏[1] 时佳[1] 

机构地区：[1]解放军第二一○医院骨科,辽宁省大连市116021

出　　处：《中国临床康复》2005年第18期230-231,i008,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:既往对先天性马蹄内翻足骨骼及神经,肌肉病理改变的研究较多,而对畸形足周围软组织变化的研究正在探索中。目的:探讨先天性马蹄内翻足的病因、病理及发病机制与细胞外基质的关系。设计:完全随机对照实验。单位:第四军医大学全军骨科研究。对象:实验于1997-11/2003-08在第四军医大学全军骨科研究所完成,实验组深筋膜标本来自于15例手术矫正的先天性马蹄内翻足患儿。男11例,女4例,均年龄9个月。对照组选择5例非神经肌肉性疾病及非胶原病死亡患儿。年龄4～21个月,平均11个月。方法:对先天性马蹄内翻足患者及非神经肌肉病变死亡小儿的足部及小腿前外侧深筋膜进行免疫组化,切片应用LeticaQ,570c彩色图像分析仪处理系统进行量化分析。主要观察指标:两组深筋膜的Ⅰ,Ⅱ,Ⅲ型胶原阳性表达及相对含量。结果:实验组足内侧Ⅰ,Ⅱ,Ⅲ型胶原含量分别为116.43±11.80,132.91±8.88,184.40±11.82;对照组分别为169.28±8.17,176.33±9.47,194.38±5.87。先天性马蹄内翻足挛缩组织Ⅰ,Ⅱ,Ⅲ型胶原含量均有增加,Ⅰ型胶原增加最显著,Ⅲ型次之,Ⅱ型增加最少。且Ⅰ,Ⅲ型胶原呈负相关。Ⅱ型胶原的阳性表达可能为伴随现象。它与Ⅰ,Ⅲ型胶原无相关性。结论:先天性马蹄内翻足细胞外基质的改变符合组织器官纤维化及一般瘢痕性纤维结缔组织增生。故认为先天性马蹄内翻足可能为足内侧的纤维化所引起。BACKGROUND:Previously,more researches are about the pathological changes in s keleton, nerve and muscular of congenital clubfoot(CCF).Researches are exploring the changes in the surrounding soft tissues of the deformed feet. OBJECTIVE: To investigate the relationships between the pathogeny,pathology a nd pathogenesis of CCF and extracellular matrix(ECM). DESIGN:A completely randomized controlled trial. SETTING:Institute of Orthopeadics of the Fourth Military Medical University. PARTICIPANTS:The study was conducted in the Institute of Orthopeadics of the Fourth Military Medical University from November 1997 to August 2003.The deep fa scia of study group was obtained from 15 CCF patients who received operative cor rections including 11 males and 4 females with an average of 9 months. Five infa nt corpses who died from non- neural muscular diseases and non- collagen disea ses aged between 4 and 21 months with an average age of 11 months were selected in the control group. METHODS:Immunohistochemical investigation was used for the assay of both stud y and control group.Slices were quantitatively analyzed by Letica Q,570c color i mage analysis and management system. MAIN OUTCOME MEASURES:Positive expressions of collagen Ⅰ ,Ⅱ and Ⅲ ,and th e relative contents in the deep fascia of both groups. RESULTS:The grey values for relative contents of collagen Ⅰ ,Ⅱ and Ⅲ in t he interior foot were 116.43± 11.80,132.91± 8.88,and 184.40± 11.82 respective ly in the study group,and 169.28± 8.17,176.33± 9.47,and 194.38± 5.87 in the c ontrol group.The contents of collagenⅠ , Ⅱ and Ⅲ increased in the contractu red tissues of CCF group with the most significant increase in collagen Ⅰ ,foll owed by collagenⅢ and the increase of collagenⅡ was least.Additionally, coll agen Ⅰ was negatively correlated with collagen Ⅲ .The positive expression of collagen Ⅱ might be an accompanying phenomenon, which had no correlation with collagen I and Ⅲ . CONCLUSION:ECM changes in CCF are accorded with fibrosis in tiss

关 键 词：马蹄足畸形 筋膜 胶原 免疫组织化学 

分 类 号：R318[医药卫生—生物医学工程]