人精子顶体反应3种检测方法的比较与评价  

Comparison of Three Methods for Evaluating Acrosome Reaction in Human Spermatozoa

作　　者：张韫[1] 谢琪璇[1] 潘善培[1] 张春雪[1] 肖銮娟[1] 彭雅林[1] 

机构地区：[1]暨南大学生殖免疫所,广东广州510632

出　　处：《中华男科学杂志》2005年第6期419-421,425,共4页National Journal of Andrology

基　　金：国家自然科学基金(30370541);广东省自然科学基金(31883);广东省科技计划项目(2003B30501)资助

摘　　要：目的:寻找简便和准确的精子顶体反应检测方法。方法:正常人精液标本液化后混合,分成6组,分别用金霉素染色法、考马斯亮蓝染色法和酸性磷酸酶检测法对孕酮诱导顶体反应前后的人精子进行形态学观察并且对顶体反应率进行数据统计。结果:经考马斯亮蓝和金霉素染色后,发生顶体反应的精子和没有发生顶体反应的精子均有明显的形态差异;经考马斯亮蓝染色、金霉素荧光染色和酸性磷酸酶测试法检验,孕酮诱导组与阴性对照组的精子顶体反应率均有明显差异(P<0.05)。结论:3种方法均可作为顶体反应的检测手段,但考马斯亮蓝染色更为简便和稳定。Objective: To find a convenient and exact method for evaluating acrosome reaction in human spermatozoa. Methods: The semen of the normal male was mixed and then divided into 6 groups. Coomassie brilliant blue (CBB) staining, chloratecycline (CTC) fluorescence staining and acid phosphatase(ACP) detection were used for morphological observation and data analysis of the acrosome status of the human sperm treated with or without progesterone. Results: There were obvious morphological differences between the acrosome-reaction and acrosome-intact spermatozoa in CBB staining and CTC fluorescence staining, and significant differences were observed between the experimental and control spermatozoa by the three methods(P< 0.05). Conclusion: All the three methods can be used to assess acrosome reaction in human spermatozoa, but Coomassie brilliant blue (CBB) staining is much more convenient and stable.

关 键 词：精子 顶体反应 考马斯亮蓝染色法 金霉素荧光染色 酸性磷酸酶 

分 类 号：R698.2[医药卫生—泌尿科学]