中药天花粉对免疫调节T细胞作用的研究  

The Effect of Trichosanthin on Immunoregulatory T Lymphocytes

作　　者：毕黎琦[1] 刘继文[1] 宋怡[1] 

机构地区：[1]白求恩医科大学第三医学院免疫内科

出　　处：《中国中西医结合杂志》1994年第1期18-20,共3页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：本研究用流式细胞仪测定了天花粉蛋白对健康人组及系统性红斑狼疮患者免疫调节Ｔ细胞（Ｔ８＋、２Ｈ４＋、４Ｂ４＋）数量的影响，同时观察了不同浓度的天花粉蛋白对人淋巴细胞ＤＮＡ合成的作用。研究发现天花粉蛋白可使Ｔ８＋细胞减少，同时增加４Ｂ４＋细胞数，促进淋巴细胞ＤＮＡ合成。提示天花粉蛋白可通过改变不同功能的免疫调节Ｔ细胞的比例，起到增强体液免疫功能的作用。The effect of trichosanthin(TCS)on immune system has drawn attentions of the investigators both at home and abroad.The purpose of this study is to evaluate the prospects of TCS on autoimmune diseases and immunodeficiency diseases by detecting the immunoregulatory T lymphocytes(T8,2H4,4B4)count and cellular DNA content under the influence of TCS with flow cytometer using indirect immunofluorescence technique and lymphocyte culture in normal subjects and systemic lupus erythematosus patients.The results showed that the percentage of T8+ cells decreased,4B4+ cells increased,2H4+ cells did not markedly change after adding TCS. The raise of DNA content in S stage was dose-dependent to TCS,especially in large dose. It is concluded that TCS could selectively inhibit T8+ cells and could obviously increase the number of 4B4+ cells.It is suggested that TCS could enhance humoral immunity through the ratio of immunoregulatory T cells.So TCS might help immunodeficiency patients such as AIDS to reestablish their immune system.

关 键 词：天花粉蛋白 免疫调节 T细胞 

分 类 号：R967[医药卫生—药理学]