高效液相色谱法测定家兔血浆中桂皮酸的浓度及其药代动力学初探  

High Performance Liquid Chromatographic Determination of Cinnamic Acid in Rabbit Plasma and Application in Study of Pharmacokinetics

作　　者：王睿[1] 孙天慧[1] 景丹[1] 陈晓辉[1] 于治国[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《色谱》2005年第3期273-275,共3页Chinese Journal of Chromatography

基　　金：国家中医药管理局中医药科学技术研究专项基金项目(国中医药科02032P22).

摘　　要：建立了采用高效液相色谱(HPLC)测定家兔血浆中桂皮酸含量的方法,并应用此法进行了桂皮酸的药代动力学研究。采用的色谱柱为KromasilC18柱(250mm×4.6mmi.d.,5μm);流动相为甲醇乙腈水冰醋酸(体积比为10∶22∶55∶0.5),流速0.8mL/min;检测波长为270nm;柱温为室温;内标物为苯丙酸。实验结果表明,低、中、高浓度的提取回收率分别为84.9%,84.4%,87.7%,方法回收率分别为98.4%,99.2%,100.1%,相对标准偏差(RSD)分别为5.5%,3.6%,3.7%,日内及日间测定值的RSD均小于6%。所建立的HPLC方法灵敏、专一、准确、精密,可作为桂皮酸在家兔体内药代动力学研究的检测手段。口服冠心苏合丸和冠心苏合胶囊后,桂皮酸在家兔体内的代谢呈一级吸收双室模型。A high performance liquid chromatographic method was developed to determine the content of cinnamic acid in rabbit plasma and the method was utilized for the study of pharmacokinetics. Cinnamic acid was separated by employing a column of Kromasil C 18 (250 mm×4.6 mm i.d., 5 μm) and a mobile phase of methanol-acetonitrile-water-glacial acetic acid (10∶22∶55∶0.5, v/v) at a flow rate of 0.8 mL/min and room temperature with UV detection at 270 nm and phenylpropionic acid as internal standard. The extraction recoveries of the spiked samples at low, middle and high levels were 84.9%, 84.4%and 87.7%, respectively, while the method recoveries were 98.4% with relative standard deviation (RSD) of 5.5%, 99.2% with RSD of 3.6%, 100.1% with RSD of 3.7% in turn. The RSDs of intra-day and inter-day were both lower than 6%. Finally, the metabolism of cinnamic acid in rabbit plasma after medication of Guanxin Suhe Wan and Guanxin Suhe Capsule fitted in a first order absorption of two-compartment model. The method was found to be sensitive, accurate and precise, and is appropriate for the determination of cinnamic acid.

关 键 词：高效液相色谱法 桂皮酸 药代动力学 冠心苏合丸 冠心苏合胶囊 

分 类 号：O658[理学—分析化学]