人骨髓基质干细胞向肝细胞分化过程中白蛋白的表达研究  

Expression of albumin during hepatocyte differentiation by human bone marrow stem cells

作　　者：李秉璐[1] 曲强[1] 赵玉沛[1] 何小东[1] 王磊[1] 陈翠珠[2] 刘振元[2] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院基本外科,100730 [2]中国医学科学院中国协和医科大学北京协和医院医学实验中心,100730

出　　处：《中华外科杂志》2005年第11期713-715,共3页Chinese Journal of Surgery

摘　　要：目的观察在体外诱导人骨髓基质干细胞向肝细胞分化过程中白蛋白的表达特征。方法从手术切除弃置的人肋骨骨髓中分离基质细胞,在含有肝细胞生长因子(HGF)、淋巴细胞抑制因子(LIF)、纤维母细胞生长因子(FGF)等条件培养基中培养细胞。应用免疫荧光方法在共聚焦显微镜下观察肝细胞特异性白蛋白染色;同时,于诱导培养后多时间点测定培养细胞产生的白蛋白水平。结果人骨髓来源的基质干细胞在条件培养基中经诱导后,分化为成熟附壁细胞;细胞胞浆内肝细胞特异性标志物白蛋白呈阳性染色;已分化细胞合成并分泌白蛋白,其表达水平随细胞分化显示时间依赖性变化特征。结论人骨髓基质干细胞经诱导培养后可向成熟肝细胞分化并具有合成和分泌白蛋白功能,可以作为临床肝细胞移植及生物人工肝等治疗终末期肝病的重要肝细胞来源。Objective To observe the expression pattern of albumin during the hepatocyte differentiation by human bone marrow stem cells in vitro. Methods Human bone marrow cells were harvested and cultured in the presence of hepatocyte growth factor (HGF), fibroblast growth factor (FGF) and lymphocyte inhibitory factor (LIF). Cells were stained immunohistochemically by albumin specific antibody and examined under a confocal microscope. Supernatant albumin level was measured biochemically on a serial time points of the culture. Results By this condition, the attached cells became mature morphologically in 1 week of culture. Hepatocyte-specific albumin could be detected in mature cells. The albumin level revealed a time-dependant change during a 4-week culture. Conclusion Human bone marrow cells could be induced to differentiate to mature hepatocytes that produce and secret albumin in vitro. These cells may contribute to a stable source of hepatocytes for clinical hepatocyte transplantation and artificial liver support system.

关 键 词：人骨髓基质干细胞 分化过程 白蛋白 表达研究 纤维母细胞生长因子 肝细胞生长因子 条件培养基 显微镜下观察 免疫荧光方法 特异性标志物 培养细胞 诱导培养 细胞分化 细胞特异性 时间依赖性 肝细胞来源 终末期肝病 生物人工肝 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]