无荚膜隐球菌CAP64对小胶质细胞凋亡的影响  

Effect of Capsule- deficient strains CAP64 on apoptosis of mice microglia cell-line (N9) cell in vitro

作　　者：杨阳[1] 廖万清[1] 徐红[1] 张玲珍[2] 赵瑾[1] 陈孙孝[1] 温海[1] 

机构地区：[1]第二军医大学长征医院皮肤科,上海200003 [2]第二军医大学长征医院诊断科,上海200003

出　　处：《解放军医学杂志》2005年第6期486-487,共2页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题(编号30370082)

摘　　要：目的　观察无荚膜隐球菌CAP64对鼠小胶质细胞凋亡的影响。方法　无荚膜隐球菌CAP64与小胶质N9细胞株共孵育,用流式细胞仪检测5个不同时段N9细胞的凋亡指数。结果　2、4、8、16、24h N9细胞平均凋亡指数分别为9 33%、12 93%、15 37%、16 30%、20 5%,与空白组、灭活组凋亡指数比较,差异均有显著性(P<0 05)。结论　无荚膜隐球菌CAP64可以诱导小胶质细胞凋亡。Objective To study the effect of capsule deficient strains CAP64 on apoptosis of mice microglia cell line (N9) cell in vitro . Methods N9 cells were co cultured with CAP64, the flow cytometry was employed to detect the N9 cellular apoptosis on different phase. Results The average apoptotic index of N9 after cultured for 2、4、8、16、24 hours with CAP64 were 9 33%、12 93%、15 37%、16 30% and 20 5%, respectively. There were significant differences between experimental group and control group. Conclusion Capsule deficient strains CAP64 can induce microglia cell line (N9) apoptosis

关 键 词：小胶质N9细胞 隐球菌属 CAP64 细胞凋亡 

分 类 号：R756[医药卫生—皮肤病学与性病学]