醛糖还原酶基因多态性同2型糖尿病肾病的相关性  

Study on the Correlation between Aldose Reductase Gene Polymorphism and Type 2 Diabetic Nephropathy

作　　者：于德民[1] 张亚文[1] 

机构地区：[1]天津医科大学代谢病医院,300070

出　　处：《天津医药》2005年第6期329-330,共2页Tianjin Medical Journal

基　　金：天津市自然科学基金资助项目(项目编号:023611211)

摘　　要：目的:研究醛糖还原酶(AR)基因启动子区C(-12)G多态性同2型糖尿病肾病(DN)发生及发展的相关性。方法:采用PCR-RFLP技术在209例2型糖尿病和84例对照组人群中筛查该位点等位基因及基因型的频率,比较在无肾病组及肾病各组中频率的差异,以推断AR基因是否参与了肾病的发生和进展。结果:G等位基因在肾病组的分布频率是87.2%,高于无肾病组的78.1%和正常对照组的77.4%;GG基因型在肾病组的分布频率是74.4%,高于无肾病组的63.8%和正常对照组的57.1%。结论:AR基因-12位点G等位基因及GG基因型是2型DN发生的独立危险因素。Objective: To study the correlation between aldose reductase (AR) gene promoter region C (-12) G polymorphism and type 2 diabetic nephropathy. Methods:C(-12) G and genotype frequency were screened in 209 type 2 diabetic patients and 84 controls by PCR-RFLP technique. Compared the difference between no nephropathy group and nephropathy group, whether AR participating in the development of nephropathy was determined. Results: The frequency of allele G in nephropathy group (87.2%) was higher than those in no nephropathy group (78.1%) and cotrol group (77.4%). The frequency of genotype GG in nephropathy group (74.4%) was higher than those in no nephropathy group (63.8%) and control group (57.1%). Conclusion: AR-12 allele G and genotype GG are independent risk factors to type 2 diabetic nephropathy.

关 键 词：2型糖尿病肾病 酶基因多态性 相关性 PCR-RFLP技术 醛糖还原酶(AR) 正常对照组 肾病(DN) 基因启动子区 等位基因 独立危险因素 AR基因 分布频率 基因型 无肾病 位点 

分 类 号：R587.2[医药卫生—内分泌] R363.21[医药卫生—内科学]