高糖对人脐静脉内皮细胞凋亡及bcl-x、bax和eNOS表达的影响  

Study on the Effects of Hyperglycemia on Human Umbilical Vein Endothelial Cell Apoptosis and bcl-x, bax and eNOS Expressions

作　　者：孟东[1] 陈樱[1] 刘德敏[1] 卫丽君[1] 马金荣[1] 张怡[1] 于德民[1] 

机构地区：[1]天津医科大学代谢病医院,300070

出　　处：《天津医药》2005年第6期335-337,共3页Tianjin Medical Journal

基　　金：天津医科大学科学基金资助(项目编号:2002KY28)

摘　　要：目的:探讨高糖对人脐静脉内皮细胞(HUVEC)凋亡的影响,及其与bcl-x、bax和eNOS表达的相关性。方法:将培养成功的HUVEC以不同浓度的葡萄糖孵育72h,提取细胞总RNA。采用半定量逆转录聚合酶链反应(SQ-RT-PCR)法检测内皮细胞bcl-x、bax以及eNOS表达。用吖啶橙/溴乙锭(AO/EB)荧光染色法进行内皮细胞凋亡定性。结果:高浓度葡萄糖(33.3mmol)可诱导HUVEC凋亡增加(P<0.01),eNOS及bcl-x表达减弱。HUVEC凋亡与eNOS表达呈负相关。结论:糖尿病状态下高血糖诱导内皮细胞凋亡,可能与bcl-x及eNOS表达减弱有关。Objective: To discuss the effects of hyperglycemia on human umbilical vein endothelial cell (HUVEC) apoptosis and analyze the relationship between apoptosis and expressions of bcl-x, bax and eNOS. Methods: HUVECs were incubated at different concentration of glucose for 72 hours, and total RNA was extracted. The expressions of bcl-x, bax and eNOS were measured by SQ-RT-PCR technique. The nature of endo thelial cells apoptosis was determined by AO/EB straining. Results: Hyperglycemia increased HUVEC apoptosis (P < 0.01) and decreased the expressions of eNOS and bcl-x. There was a negative correlation between HUVEC apoptosis and the eNOS expression. Conclusion: Hyperglycemia can induce ECs apoptosis in DM, and it may be related to the decrease of eNOS and bcl-x expressions.

关 键 词：内皮细胞凋亡 BCL-X NOS表达 半定量逆转录聚合酶链反应 高糖 BAX 人脐静脉内皮细胞 HUVEC 高浓度葡萄糖 荧光染色法 不同浓度 总RNA eNOS BAX 相关性 72h 溴乙锭 吖啶橙 负相关 高血糖 糖尿病 减弱 

分 类 号：R543.5[医药卫生—心血管疾病] R651.15[医药卫生—内科学]