子宫内膜异位症患者腹腔液中IFN-γ和IL-4水平的关系  

Study on the Levels of Interfron-r and Interleukin-4 Secreted by T Helper Cells inPeritoneal Fluid in Endometriosis Patients

作　　者：任健俐[1] 尹利荣[1] 荣卓[1] 王宇全[1] 米淑玲[1] 张淑敏[1] 

机构地区：[1]天津医科大学第二医院,300211

出　　处：《天津医药》2005年第6期351-352,共2页Tianjin Medical Journal

摘　　要：目的:探讨子宫内膜异位症(内异症)患者腹腔液中Th细胞分泌γ干扰素(IFN-γ)和白细胞介素(IL)-4水平与内异症发病的关系。方法:选取经手术后病理证实为内异症的24例患者为内异症组;以子宫肌瘤或卵巢良性肿瘤患者24例为对照组。分别分离、培养2组腹腔液的Th细胞,用酶联免疫吸附试验(ELISA)检测其培养的上清液中IFN-γ和IL-4水平,并计算IFN-γ/IL-4的比值。结果:内异症组腹腔液Th细胞培养上清液IFN-γ的水平低于对照组(P<0.01);而IL-4的水平高于对照组(P<0.05)。内异症组IFN-γ/IL-4的比值低于对照组(P<0.01)。结论:内异症患者腹腔液Th细胞培养上清液中IFN-γ/IL-4比值的变化揭示Th1/Th2的失衡状态,可导致异位内膜逃避免疫监视,造成异位内膜种植。Objective: To investigate the relationship between the pathogenesis and the levels of Interfron-r and Interleukin-4 secreted by T helper (Th) cells in peritoneal fluid in endometriosis. Methods: Twenty-four women with pathological diagnosed endometriosis after operation were selected as endometriosis group and 24 with leiomyoma or benign ovarian tumor were selected as control group. Th cells in peritoneal fluid were isolated and cultured, the levels of IFN-r and IL-4 in culture media were determined by enzyme-linked immunosorbent assay (ELISA) and the ratios of IFN-r/IL-4 were calculated. Results: The level of IFN-r secreted by Th cells in endometriosis group was significantly lower than that of control group (P < 0.01) and the level of IL-4 in endometriosis group was higher than that of control group (P < 0.05). The ratio of IFN-r/IL-4 of endometriosis group was lower than that of control group (P < 0.01). Conclusion: The change of IFN-r/IL-4 ratio in endometriosis approves the imbalance state of Th 1/ Th 2, which leads to endometriosis escaping from immune surveillance, and results in ectopic membrane implantation.

关 键 词：子宫内膜异位症 腹腔液 酶联免疫吸附试验(ELISA) 水平 IFN-Γ/IL-4 细胞培养上清液 Th1/Th2 异位内膜 内异症 白细胞介素 对照组 γ干扰素 细胞分泌 病理证实 肿瘤患者 子宫肌瘤 免疫监视 手术后 比值 

分 类 号：R711.71[医药卫生—妇产科学] R512.6[医药卫生—临床医学]