毛细胞前体细胞的分离和鉴定  

Isolation and Identification of Hair Cell Progenitors

作　　者：时文杰[1] 时利[2] 翟所强[2] 杨伟炎[2] 

机构地区：[1]天津市第一中心医院耳鼻咽喉科,300192 [2]中国人民解放军总医院耳鼻咽喉研究所

出　　处：《天津医药》2005年第6期366-368,F003,共4页Tianjin Medical Journal

基　　金：中国博士后科学基金资助课题(项目编号:中博基2002第17号)

摘　　要：目的:证实哺乳类耳蜗大上皮嵴和小上皮嵴分别能转化为内、外毛细胞。方法:采用体外培养wistar大鼠内耳基底膜,进行了大、小上皮嵴的定位和鉴定。结果:基底膜培养中,小上皮嵴细胞都能被毛细胞的早期标志肌球蛋白(myosinVIIa)染色,表明毛细胞分化,同时,在培养基中发现一些球囊型结构的细胞,细胞增殖标志(BrdU)、上皮细胞标志(ZO1)免疫组化阳性,表明该球囊不仅具有增殖能力,而且具有上皮特性。结论:建立了新生大鼠耳蜗铺片培养技术,识别和定位毛细胞前体细胞(即小上皮嵴和大上皮嵴区域),证明了小上皮嵴细胞和大上皮嵴细胞都具有转变成为毛细胞的潜能。Objective: To establish the possibility of translation from greater epithelial ridge (GER) and lesser epithelial ridge (LER) of the mammalia cochlea into inner and outer hair cells respectively. Methods: Basememt membrane (BM) of wistar rats were cultured in vitro and orientation and identification of GER and LER were taken. Results: LER were dyed by myosin VIIa which proved hair cells differentiation in BM culture. Some saccule-like cells were found in the culture medium and the results of BrdU and ZO1 were positive by immunohistochemistry which proved proliferative capabilities and epithelium characteristics of these cells. Conclusion: Culture technique of newborn rats cochlea is successfully built, hair cell progenitors (LER and GER) are identified and located, and the translation from LER and GER into hair cells is established.

关 键 词：前体细胞 鉴定 WISTAR大鼠 分离 外毛细胞 体外培养 肌球蛋白 早期标志 细胞分化 细胞增殖 免疫组化 上皮细胞 增殖能力 培养技术 耳蜗铺片 新生大鼠 哺乳类 基底膜 膜培养 培养基 

分 类 号：R581.1[医药卫生—内分泌] R-332[医药卫生—内科学]