特异性COX-2抑制剂对压力负荷性心肌肥厚大鼠左心室重构的影响  

The influence of selective cyclooxygenase-2 inhibitor on left ventricular reconstitution in rats with pressure overloaded myocardial hypertrophy

作　　者：梁子敬[1] 曾量波[1] 叶政[2] 陈国勤[3] 王鹏鲲[4] 卢敏俊 

机构地区：[1]广州医学院第一附属医院急诊科,广州510120 [2]广州医学院第一附属医院B超室,广州510120 [3]广州医学院第一附属医院病理科,广州510120 [4]广州医学院第一附属医院检验科,广州510120 [5]广州市第三人民医院

出　　处：《中华急诊医学杂志》2005年第6期479-481,共3页Chinese Journal of Emergency Medicine

摘　　要：目的观察特异性COX2抑制剂对大鼠慢性压力负荷性心肌肥厚过程中左室重构的影响。方法将SD大鼠随机分3组:模型对照组、模型组和实验组,模型组和实验组按腹主动脉缩窄致压力负荷性心肌肥厚制作大鼠模型。实验组加用特异性COX2抑制剂rofecoxib干预,20周用高频超声测定左心室结构和功能,心肌组织胶原Masson染色,测定总胶原容积百分比(CVF T)和无冠状动脉小血管视野胶原容积百分比(CVF NV)。结果模型组左室壁增厚,特别是室间隔增厚明显,左室重量增加(P<0.01),左室腔扩大。实验组左室舒张内径(LVDD)及LVM较模型组降低(P<0.01)。模型组CVF T和CVF NV明显增加(P<0.01),实验组则减少(P<0.01),与模型对照组无差异(P>0.05)。结论COX2参与了心肌胶原重塑的过程。特异性COX2抑制剂有抗心室重构作用,可能与抗心肌纤维化的作用有关。Objective To investigate the influence of selective cyclooxygenase-2 inhibitor on rats with pressure overloaded myocardial hypertrophy. Methods Eighteen male SD rats were randomly divided into 3 groups: sham operation group(n=5),operation group(n=7) and rofecoxib group(n=6).Myocardial hypertrophy was induced by gradually constricting the abdominal aorta in rats.After 16 weeks, rofecoxib group was treated with rofecoxib and a selective COX-2 inhibitor(20 mg·kg^(-1)·d^(-1))in drinking water for 4 weeks. All rats were assessed for progressive changes in left ventricular (LV) structures and functions with high-frequency ultrasound at 20 weeks after operation.Myocardial collagen was stained with Masson and CVF-T and CVF-NV were analyzed using a computer assisted procedure. Results Operation rats had increased wall thickness,internal diameter,mass in LV and had decreased FS.LVDD and LVM in rofecoxib group. CVF-T and CVF-NV increased significantly in operation group,and there was no difference between rofecoxib group and sham operation group.Conclusion COX-2 products contribute to myocardial collagen remodeling. The selective COX-2 inhibitor may prevent myocardial fibrosis in pressure overloaded rats.

关 键 词：特异性COX-2抑制剂 压力负荷性 心肌肥厚 左心室重构 ROFECOXIB Masson染色 腹主动脉缩窄 结构和功能 室间隔增厚 心肌纤维化 实验组 左室重构 SD大鼠 大鼠模型 超声测定 心肌组织 冠状动脉 室壁增厚 左室重量 心肌胶原 

分 类 号：R541[医药卫生—心血管疾病]