BoLA-DQB基因上游调控区多态性研究  

Polymorphism in Upstream Regulatory Region of BoLA-DQB Genes

作　　者：王昆[1] 孙东晓[1] 徐如海[1] 王志刚[2] 张沅[1] 

机构地区：[1]中国农业大学动物科学技术学院,北京100094 [2]农业部全国畜牧兽医总站畜禽牧草种质资源保存利用中心,北京100094

出　　处：《畜牧兽医学报》2005年第6期521-525,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展计划(973)项目(TG2000016105);国家自然科学基金项目(30170676)

摘　　要：通过PCR扩增和克隆测序,对BoLA DQB 基因上游调控区的多态性进行研究。对BoLA DQB 基因ex on2的14种等位基因上游调控区序列进行同源性比较,结果表明:每个DQB·exon2 等位基因均与2 种DQB URR相连锁,在14种BoLA DQB·exon2等位基因上游共检测到15种DQB URR序列。这些序列中均存在与基因表达调控有关的W box 、X box、Y box、CCAAT box以及类TATA box调控元件,且遵循严格的空间顺序。其中W box 、X box 、Y box及类TATA box是多态的,CCAAT box是保守的,在这些调控元件之间也存在多态座位。这些多态座位的存在有可能对结构基因的表达水平产生影响。Upstream regulatory region of 14 alleles were studied for polymorphism in BoLA-DQB loci. URRs of each allele were amplified by the polymerase chain reaction. The PCR products were cloned and sequenced. Alignment of the URR sequences showed that each DQB·exon2 alleles linked with 2 different URR sequences, thus, of 14 DQB alleles analyzed, a total of 15 different DQB-URR were found. Regulatory elements W box,X box,Y box,CCAAT box and TATA-like box which were associated with the expression of structural gene were found in all the URR sequences. W box,X box,Y box and TATA-like box were polymorphic, CCAAT box was conserved. Polymorphic sites also existed between the regulatory elements. This perhaps affected the level of expression of structural genes.

关 键 词：上游调控区 B基因 多态性研究 等位基因 基因表达调控 box 调控元件 多态座位 PCR扩增 同源性比较 克隆测序 结构基因 序列 

分 类 号：S852.65[农业科学—基础兽医学] S881.2[农业科学—兽医学]