猪Pit-1基因的多态性研究  

Analyses of the Polymorphisms of Pig Pit-1 Gene

作　　者：庞瑾[1] 李宏滨[1] 郑友民[2] 杜立新[1] 

机构地区：[1]中国农业科学院畜牧研究所,北京100094 [2]中国农业科学院农业质量标准与检测技术研究所,北京100081

出　　处：《畜牧兽医学报》2005年第6期531-535,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(39970542);国家重点基础研究发展规划"973"项目(TG2000016104)

摘　　要：选取7个中国地方猪种和3 个国外引入猪种共473 头,应用PCR SSCP技术在7 个中国地方猪种中检测到1个Pit 1基因第4外显子上的突变;应用PCR RFLP技术在3个国外引入猪种中,扩增长度为1 747 bp的片段中检测到1个RsaⅠ限制性内切酶的多态酶切位点。遗传多态性分析结果表明:外显子4上的突变,在7个中国地方猪种中是A型等位基因和AA基因型频率占优势。其中沂蒙黑猪和巴马小型猪处于Hardy Weinberg平衡;北京黑猪、沂蒙黑猪、巴马小型猪、滇南小耳猪和香猪处于中度多态。RsaⅠ酶切多态位点的突变在3个国外引入猪种中也是A型等位基因和AA基因型频率占优势。其中大白猪和杜洛克猪处于Hardy Weinberg平衡;3 个国外引入猪种的多态信息含量均为中度多态。Seven Chinese local pig breeds, including Beijing Black pig, Yimeng Black pig, Laiwu pig, Bama Small pig, Diannan Small-ear pig, Wuzhishan pig, Xiang pig, and three foreign breeds, including Large White, Landrace and Duroc were used as experimental examples. A mutation in exon4 of pig Pit-1 gene in seven Chinese local breeds was found by using PCR-SSCP analysis. A RsaⅠ restriction enzyme polymorphic site in three foreign introduced breeds was detected by using PCR-RFLP analysis. The result of population genetics analyses showed that in the mutation of exon4, the frequency of A allele and AA genotype were significantly higher than B allele and BB genotype in the most of seven Chinese local breeds. A chi-square analysis suggested that Beijing Black pig and Bama Small pig reached Hardy-Weinberg equilibrium(P>0.05), Beijing Black pig, Yimeng Black pig, Bama Small pig, Diannan Small-ear pig and Xiang pig had intermediate polymorphism(0.25<PIC<0.5). For RsaⅠ-RFLP, the frequency of A allele and AA genotype were higher than B allele and BB genotype. Large White and Duroc reached Hardy-Weinberg equilibrium(P>0.05); and all of the three foreign introduced breeds had intermediate polymorphism(0.25<PIC<0.5).

关 键 词：Hardy-Weinberg平衡 多态性研究 PCR-SSCP技术 PCR-RFLP技术 中国地方猪种 基因型频率 巴马小型猪 限制性内切酶 多态信息含量 等位基因 沂蒙黑猪 遗传多态性 酶切位点 分析结果 北京黑猪 多态位点 杜洛克猪 外显子 Rsa 

分 类 号：S829.2[农业科学—畜牧学] S852.23[农业科学—畜牧兽医]