猪雌性生殖道黏膜显微和超微结构观察  

Observation on the Microstructure and Ultrastructure of Genital Tract Mucosa of Sow

作　　者：金光明[1] 杨倩[2] 王珏[1] 李升和[1] 刘胜兵[2] 李玉磊[2] 

机构地区：[1]安徽科技学院动物科学学院,凤阳233100 [2]南京农业大学动物医学院,南京210095

出　　处：《畜牧兽医学报》2005年第6期612-615,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省自然科学基金资助项目(BK2002111 );安徽省自然科学基金项目(04023071 )

摘　　要：应用组织学方法和电镜技术研究了雌性成年猪生殖道黏膜显微和超微结构的特征。试验选用成年长白×大约克夏二元杂交母猪20头,通过光镜和透射电镜分别观察了母猪的输卵管、子宫和阴道的黏膜结构特征。结果显示成年母猪的生殖道黏膜由上皮、固有膜和黏膜肌层等构成,输卵管、子宫角、子宫体和子宫颈黏膜的上皮为单层柱状上皮,上皮的游离面含有纤毛和微绒毛,固有膜和上皮间含有丰富的淋巴细胞。淋巴细胞在上皮内移行过程中由胞质伸出许多伪足,淋巴细胞的胞质中主要含有线粒体和高尔基体。母猪生殖道黏膜内的淋巴细胞在某些部位聚集形成淋巴细胞群,特别是子宫角和子宫颈黏膜中较多,淋巴细胞向子宫腔内移行呈现“腔排”现象。结果显示猪雌性生殖道黏膜中含有较多的淋巴细胞,生殖道黏膜可能是黏膜免疫的诱导位点。The microstructure and ultrastructure of genita tract mucosa in sow were observed by histological method and electron microscopic technique. Twenty sows of Landrace× Yorkshire two-way crossbred were selected . The tissue structure of oviduct, uterus and vagina mucosa in sow were respectively observed by transmission electron microscopy(SEM) and light microscopy(LM). The results showed that the genital tract mucosa was composed of epithelia, lamina propria and muscularis mucosa. The cilium and microvilli were observed on the simple columnar epithelium in oviduct and uterus. Some lymphocytes were found in the epithelia and lamina propria, and passed through the epithelia and arrived the epithelial surface. The pseudopod of lymphocytoplasm were jutted out. The mitochondria and Golgi complex were rich in the lymphocytoplasm. Lymphocyte aggregation of genital mucosa were formed in the stroma, lymphocytes in cervix mucosa arrived to the epithelial surface, like the phenomenon “lumen excretion” . Our studies revealed that genital mucosa in sow may be the induce site of mucosal immune, since numerous lymphocytes distributed in it.

关 键 词：雌性生殖道 超微结构观察 显微 淋巴细胞 二元杂交母猪 电镜技术 大约克夏 透射电镜 结构特征 高尔基体 黏膜免疫 输卵管 子宫角 子宫颈 上皮 成年 组织学 微绒毛 线粒体 细胞群 显示 胞质 移行 长白 阴道 位点 

分 类 号：S824.1[农业科学—畜牧学] S946.11[农业科学—畜牧兽医]