用荧光半定量RT-PCR检测蚊溴氰菊酯抗性  

Detection of deltamethrin resistance in Culex pipiens pallen with semi-quantitative fluorescent RT-PCR assay

作　　者：马磊[1] 沈波[1] 李秀兰[1] 胡小邦[1] 朱昌亮[1] 

机构地区：[1]南京医科大学病原生物学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2005年第7期447-449,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30170835);南京师范大学生命科学学院江苏省资源生物技术重点实验室开放研究课题资助项目(164070300801)

摘　　要：目的:建立蚊对菊酯类杀虫剂抗性的PCR检测方法。方法:应用荧光半定量RT鄄PCR,检测经SSH结合cDNA芯片分离鉴定的淡色库蚊对溴氰菊酯抗性相关基因NYD鄄Tr在抗性品系与敏感品系中的表达水平。结果:经荧光半定量RT鄄PCR检测,NYD鄄Tr进入指数期的循环数抗性品系早于敏感品系,抗性品系组比敏感品系组先进入指数增长期。结论:以NYD鄄Tr作为靶基因,荧光半定量RT鄄PCR可区分淡色库蚊对溴氰菊酯抗性品系与敏感品系,亦即可检测淡色库蚊溴氰菊酯抗性。Objective: To establish a semi-quantitative fluorescent RT-PCR assay for detection of deltamethrin resistance in mosquitoes. Methods: Semi-quantitative fluorescent RT-PCR were used to detect the expression level of NYD-Tr gene which was associated with deltamethrin resistance in Culex pipiens pallens. Results: RT-PCR assays showed that the relative expression levels of NYD-Tr mRNA were higher in deltamethrin resistant strains than in susceptible strains of Cx.pipiens pallens. Conclusion: Results of this study demonstrate that semi-quantitative fluorescent RT-PCR analysis is probably an effective tool for detecting deltamethrin resistance in Cx.pipiens pallens.

关 键 词：淡色库蚊 抗药性 溴氰菊酯 荧光半定量RT-PCR 

分 类 号：S767.16[农业科学—森林保护学]